概述

  • 产品名称
    Anti-RNase H1抗体
  • 描述
    小鼠单克隆抗体to RNase H1
  • 经测试应用
    适用于: WB, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: AACKAIEQAK TQNINKLVLY TDSMFTINGI TNWVQGWKKN GWKTSAGKEV INKEDFVALE RLTQGMDIQW MHVPGHSGFI GNEEADRLAR EGAKQSED, corresponding to amino acids 189-287 of Human RNase H1

  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    单克隆
  • 同种型
    IgG2a
  • 轻链类型
    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab56560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 32 kDa.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 相关性
    Rnase H1 specifically degrades the RNA of RNA-DNA hybrids.
  • 细胞定位
    Cytoplasmic
  • 数据库链接
  • 别名
    • H1RNA antibody
    • MGC108918 antibody
    • Ribonuclease H type II antibody
    • RibonucleaseH1 antibody
    • RNaseH1 antibody
    • RNH1 antibody
    see all

图片

  • All lanes : Anti-RNase H1 antibody (ab56560) at 1 µg/ml

    Lane 1 : Human fibroblasts whole cell lysate - untransfected
    Lane 2 : Human fibroblasts whole cell lysate - transfected with RnaseH construct

    Lysates/proteins at 25 µg per lane.

    Secondary
    HRP-conjugated sheep anti-mouse IgG polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 32 kDa
    Observed band size : 59 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    The plasmid has a GFP tag which adds ~27kDa to the protein, expected size ~59kDa.

    See Abreview



  • Predicted band size : 32 kDa
    RNase H1 antibody (ab56560) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • Overlay histogram showing Jurkat cells stained with ab56560 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56560, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

文献

This product has been referenced in:
  • Jangi M  et al. SMN deficiency in severe models of spinal muscular atrophy causes widespread intron retention and DNA damage. Proc Natl Acad Sci U S A 114:E2347-E2356 (2017). WB ; Human . Read more (PubMed: 28270613) »
  • Akman G  et al. Pathological ribonuclease H1 causes R-loop depletion and aberrant DNA segregation in mitochondria. Proc Natl Acad Sci U S A 113:E4276-85 (2016). Read more (PubMed: 27402764) »

See all 5 Publications for this product

客户评价及客户问答

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Human Cell lysate - whole cell (fibroblasts)
Specification
fibroblasts
Treatment
RnaseH over-expression, collected 48H post transfection
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

提交于 Apr 28 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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