兔多克隆抗体to RNA terminal phosphate cyclase-like 1 - Carboxyterminal end
Synthetic peptide selected from the center region of human RCL1, conjugated to KLH.
- Mouse brain and HepG2 tissue lysates
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Concentration information loading...
Protein G purified
ab71345 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/100 - 1/500. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
Does not have cyclase activity. Plays a role in 40S ribosomal subunit biogenesis in the early pre rRNA processing steps at sites A0, A1 and A2 that are required for proper maturation of the 18S RNA.
- RCL1 antibody
- RNA 3' terminal phosphate cyclase like protein antibody
- RNA cyclase homolog antibody
Western blot - RNA terminal phosphate cyclase-like 1 antibody - Carboxyterminal end (ab71345)
All lanes :
Anti-RNA terminal phosphate cyclase-like 1 antibody - Carboxyterminal end (ab71345) at 1/60 dilutionLane 1 :
mouse brain tissue lysateLane 2 :
HepG2 tissue lysate
Lysates/proteins at 35 µg per lane.Predicted band size:
41 kDaObserved band size:
41 kDa (why is the actual band size different from the predicted?
)Additional bands at:
75 kDa. We are unsure as to the identity of these extra bands.
has not yet been referenced specifically in any publications.
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