重组Anti-RNA polymerase II RPB1抗体[EPR1509Y] - ChIP Grade (ab76123)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1509Y] to RNA polymerase II RPB1 - ChIP Grade
- Suitable for: WB, ChIP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-RNA polymerase II RPB1抗体[EPR1509Y] - ChIP Grade
参阅全部 RNA polymerase II RPB1 一抗 -
描述
兔单克隆抗体[EPR1509Y] to RNA polymerase II RPB1 - ChIP Grade -
宿主
Rabbit -
经测试应用
适用于: WB, ChIPmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human RNA polymerase II RPB1 aa 100-200 (N terminal). The exact sequence is proprietary.
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阳性对照
- HeLa whole cell lysate (ab150035).
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR1509Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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ChIP Related Products
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76123于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/1000. Detects a band of approximately 270 kDa (predicted molecular weight: 217 kDa).
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ChIP |
Use at an assay dependent concentration.
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说明 |
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WB
1/500 - 1/1000. Detects a band of approximately 270 kDa (predicted molecular weight: 217 kDa). |
ChIP
Use at an assay dependent concentration. |
靶标
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相关性
RPB1 is a subunit of RNA polymerase II which catalyses transcription of RNA from DNA. RPB1 forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. -
细胞定位
Nuclear -
数据库链接
- Entrez Gene: 5430 Human
- Omim: 180660 Human
- SwissProt: P24928 Human
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别名
- DNA directed RNA polymerase II subunit RPB1 antibody
- hRPB220 antibody
- hsRPB1 antibody
see all
图片
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Anti-RNA polymerase II RPB1 antibody [EPR1509Y] - ChIP Grade (ab76123) at 1/2000 dilution + HeLa cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 217 kDa
Observed band size: 270 kDa why is the actual band size different from the predicted? -
Chromatin was prepared from 293T cells according to the Abcam Dual-X-ChIP protocol*. Cells were fixed with 1.5 mM EGS for 30mins and then formaldehyde for 10min.
The ChIP was performed with 25 µg of chromatin, 5 µg of ab76123 (red), or 5 µg of rabbit normal IgG ab172730 (gray) and 20 µl of Protein A/G sepharose beads. The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci).
Primers and probes are commercial primers from Paper
*http://www.abcam.com/resources?keywords=X%20ChIP%20protocol
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (14)
ab76123 被引用在 14 文献中.
- Fan Y et al. PRKDC promotes hepatitis B virus transcription through enhancing the binding of RNA Pol II to cccDNA. Cell Death Dis 13:404 (2022). PubMed: 35468873
- Fasano C et al. Identifying novel SMYD3 interactors on the trail of cancer hallmarks. Comput Struct Biotechnol J 20:1860-1875 (2022). PubMed: 35495117
- Tian Y et al. Transcriptome-wide stability analysis uncovers LARP4-mediated NF?B1 mRNA stabilization during T cell activation. Nucleic Acids Res 48:8724-8739 (2020). PubMed: 32735645
- NandyMazumdar M et al. Looping of upstream cis-regulatory elements is required for CFTR expression in human airway epithelial cells. Nucleic Acids Res 48:3513-3524 (2020). PubMed: 32095812
- Cao L et al. Genotoxic stress-triggered ß-catenin/JDP2/PRMT5 complex facilitates reestablishing glutathione homeostasis. Nat Commun 10:3761 (2019). PubMed: 31434880