Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2)抗体(ab70324)

概述

  • 产品名称Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2)抗体
    参阅全部 RNA polymerase II CTD repeat YSPTSPS 一抗
  • 描述
    兔多克隆抗体to RNA polymerase II CTD repeat YSPTSPS (phospho S2)
  • 经测试应用适用于: IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Cow, Dog, Pig, Chimpanzee, Drosophila melanogaster, Plants, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • 免疫原

    Synthetic peptide corresponding to Human RNA polymerase II CTD repeat

    YSPTSPS

    (C terminal) (phospho S2).
    Sequence:

    YSPTSPS


    Database link: P24928

  • 阳性对照
    • Whole cell lysate from HeLa cells

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab70324 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2500 - 1/20000. Detects a band of approximately 270 kDa.
ELISA Use at an assay dependent concentration.

靶标

  • 功能DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Acts as an RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome.
  • 序列相似性Belongs to the RNA polymerase beta' chain family.
  • 结构域The C-terminal domain (CTD) serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing.
  • 翻译后修饰The tandem heptapeptide repeats in the C-terminal domain (CTD) can be highly phosphorylated. The phosphorylation activates Pol II. Phosphorylation occurs mainly at residues 'Ser-2' and 'Ser-5' of the heptapeptide repeat and is mediated, at least, by CDK7 and CDK9. CDK7 phosphorylation of POLR2A associated with DNA promotes transcription initiation by triggering dissociation from DNA. Phosphorylation also takes place at 'Ser-7' of the heptapeptide repeat, which is required for efficient transcription of snRNA genes and processing of the transcripts. The phosphorylation state is believed to result from the balanced action of site-specific CTD kinases and phosphatases, and a 'CTD code' that specifies the position of Pol II within the transcription cycle has been proposed. Dephosphorylated by the protein phosphatase CTDSP1.
    Among tandem heptapeptide repeats of the C-terminal domain (CTD) some do not match the Y-S-P-T-S-P-S consensus, the seventh serine residue 'Ser-7' being replaced by a lysine. 'Lys-7' in these non-consensus heptapeptide repeats can be alternatively acetylated, methylated and dimethylated. EP300 is one of the enzyme able to acetylate 'Lys-7'. Acetylation at 'Lys-7' of non-consensus heptapeptide repeats is associated with 'Ser-2' phosphorylation and active transcription. It may regulate initiation or early elongation steps of transcription specially for inducible genes.
    Methylated at Arg-1810 prior to transcription initiation when the CTD is hypophosphorylated, phosphorylation at Ser-1805 and Ser-1808 preventing this methylation. Symmetrically or asymmetrically dimethylated at Arg-1810 by PRMT5 and CARM1 respectively. Symmetric or asymmetric dimethylation modulates interactions with CTD-binding proteins like SMN1/SMN2 and TDRD3. SMN1/SMN2 interacts preferentially with the symmetrically dimethylated form while TDRD3 interacts with the asymmetric form. Through the recruitment of SMN1/SMN2, symmetric dimethylation is required for resolving RNA-DNA hybrids created by RNA polymerase II, that form R-loop in transcription terminal regions, an important step in proper transcription termination. CTD dimethylation may also facilitate the expression of select RNAs. Among tandem heptapeptide repeats of the C-terminal domain (CTD) some do not match the Y-S-P-T-S-P-S consensus, the seventh serine residue 'Ser-7' being replaced by a lysine. 'Lys-7' in these non-consensus heptapeptide repeats can be alternatively acetylated, methylated and dimethylated. Methylation occurs in the earliest transcription stages and precedes or is concomitant to 'Ser-5' and 'Ser-7' phosphorylation.
    Ubiquitinated by WWP2 leading to proteasomal degradation (By similarity). Following UV treatment, the elongating form of RNA polymerase II (RNA pol IIo) is ubiquitinated UV damage sites without leading to degradation: ubiquitination is facilitated by KIAA1530/UVSSA and promotes RNA pol IIo backtracking to allow access to the nucleotide excision repair machinery.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • DNA directed RNA polymerase II A antibody
    • DNA-directed RNA polymerase II largest subunit RNA polymerase II 220 kd subunit antibody
    • DNA-directed RNA polymerase II subunit A antibody
    • DNA-directed RNA polymerase II subunit RPB1 antibody
    • DNA-directed RNA polymerase III largest subunit antibody
    • hRPB220 antibody
    • hsRPB1 antibody
    • POLR2 antibody
    • Polr2a antibody
    • POLRA antibody
    • Polymerase (RNA) II (DNA directed) polypeptide A 220kDa antibody
    • Polymerase (RNA) II (DNA directed) polypeptide A antibody
    • RNA polymerase II subunit B1 antibody
    • RNA-directed RNA polymerase II subunit RPB1 antibody
    • RPB1 antibody
    • RPB1_HUMAN antibody
    • RPBh1 antibody
    • RpIILS antibody
    • RPO2 antibody
    • RPOL2 antibody
    see all

Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody 图像

  • Serially diluted ab70324 was bound to immobilised phospho- or control peptides (1 microgram per mL). The antibody was detected by HRP-labelled goat anti-rabbit IgG (ab97080; diluted 50000 times) and signal was developed by TMB substrate.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right) tissues labelling RNA polymerase II CTD repeat YSPTSPS (phospho S2) with ab70324 at 1/500 (0.4µg/ml). Detection: DAB.
  • All lanes : Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody (ab70324) at 0.02 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells with no peptide
    Lane 2 : Whole cell lysate from HeLa cells with non-phosphorylated peptide
    Lane 3 : Whole cell lysate from HeLa cells with phosphorylated S2 peptide
    Lane 4 : Whole cell lysate from HeLa cells with phosphorylated S5 peptide

    Lysates/proteins at 50 µg per lane.


    Observed band size : 270 kDa (why is the actual band size different from the predicted?)

Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody (ab70324)参考文献

ab70324 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam and thank you for your patience while I consulted my coleagues about your enquiry. Below is the response that I received from a colleague: "I would suggest using the non phosphorylated form of Pol2 as a positive control f...

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Please accept my apologies for misunderstanding your original email. I have to admit that I am no expert in ChIP assays but I have sent an email to ask my colleagues and I will let you know what they say. I see no reason why you could not use S...

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Thank you for contacting Abcam about ab70324. As long as there is a sufficient concentration of your sample potein present, the anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody should work. As it has not been tested in ChIP, we...

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