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Synthetic peptide corresponding to Human RNA polymerase II CTD repeat
YSPTSPS(C terminal) (phospho S2).
Our Abpromise guarantee covers the use of ab70324 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2500 - 1/20000. Detects a band of approximately 270 kDa.|
|ELISA||Use at an assay dependent concentration.|
Serially diluted ab70324 was bound to immobilised phospho- or control peptides (1 microgram per mL). The antibody was detected by HRP-labelled goat anti-rabbit IgG (ab97080; diluted 50000 times) and signal was developed by TMB substrate.
ab70324 has not yet been referenced specifically in any publications.