重组Anti-RNA polymerase beta抗体[EPR18704] (ab191598)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18704] to RNA polymerase beta
- Suitable for: IP, WB
- Reacts with: Escherichia coli
Related conjugates and formulations
概述
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产品名称
Anti-RNA polymerase beta抗体[EPR18704]
参阅全部 RNA polymerase beta 一抗 -
描述
兔单克隆抗体[EPR18704] to RNA polymerase beta -
宿主
Rabbit -
经测试应用
适用于: IP, WBmore details -
种属反应性
与反应: Escherichia coli -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB & IP: E.coli whole cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR18704 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab191598于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IP |
1/30.
|
|
WB | (2) |
1/2000. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa).
|
说明 |
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IP
1/30. |
WB
1/2000. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa). |
靶标
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相关性
RNA polymerase beta (rpoB) is the ß subunit of the bacterial RNA polymerase and is the subunit which possesses the polymerase activity. This protein is a highly conserved enzyme contained by many bacteria and has been reported as a useful marker for studies in bacetrial identification and ecology. -
细胞定位
Cell Membrane and Cytoplasmic -
数据库链接
- Entrez Gene: 948488 Escherichia coli
- SwissProt: P0A8V2 Escherichia coli
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别名
- groN antibody
- nitB antibody
- rif antibody
see all
图片
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Anti-RNA polymerase beta antibody [EPR18704] (ab191598) at 1/2000 dilution + E.coli whole cell lysates at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 150 kDa
Observed band size: 150 kDa
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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RNA polymerase beta was immunoprecipitated from 1mg of E.coli whole cell lysates with ab191598 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab191598 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.
Lane 1: E.coli whole cell lysates 10ug (Input). Lane 2: ab191598 IP in E.coli whole cell lysates. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab191598 in E.coli whole cell lysates.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (7)
ab191598 被引用在 7 文献中.
- Plaza N et al. Identification of a Family of Vibrio Type III Secretion System Effectors That Contain a Conserved Serine/Threonine Kinase Domain. mSphere 6:e0059921 (2021). PubMed: 34346702
- Zhang X et al. Bis-molybdopterin guanine dinucleotide modulates hemolysin expression under anaerobiosis and contributes to fitness in vivo in uropathogenic Escherichia coli. Mol Microbiol 116:1216-1231 (2021). PubMed: 34494331
- Liao J et al. Bacterial quorum sensing quenching activity of Lysobacter leucyl aminopeptidase acts by interacting with autoinducer synthase. Comput Struct Biotechnol J 19:6179-6190 (2021). PubMed: 34900131
- Stårsta M et al. RHS-elements function as type II toxin-antitoxin modules that regulate intra-macrophage replication of Salmonella Typhimurium. PLoS Genet 16:e1008607 (2020). PubMed: 32053596
- Klein K et al. Deprivation of the Periplasmic Chaperone SurA Reduces Virulence and Restores Antibiotic Susceptibility of Multidrug-Resistant Pseudomonas aeruginosa. Front Microbiol 10:100 (2019). PubMed: 30846971
- Virtanen P et al. Class II contact-dependent growth inhibition (CDI) systems allow for broad-range cross-species toxin delivery within the Enterobacteriaceae family. Mol Microbiol 111:1109-1125 (2019). PubMed: 30710431
- Høyland-Kroghsbo NM et al. Temperature, by Controlling Growth Rate, Regulates CRISPR-Cas Activity in Pseudomonas aeruginosa. MBio 9:N/A (2018). PubMed: 30425154