概述

  • 产品名称Anti-RMI2抗体
    参阅全部 RMI2 一抗
  • 描述
    兔多克隆抗体to RMI2
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Cow, Pig, Chimpanzee, Macaque Monkey, Gorilla, Chinese Hamster, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human RMI2.

  • 阳性对照
    • This antibody gave a positive signal in both HEK293 and MCF7 whole cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab108491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).

靶标

  • 功能Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. The complex is therefore essential for the stability, localization, and function of complexes containing BLM. In the RMI complex, it is required to target BLM to chromatin and stress-induced nuclear foci and mitotic phosphorylation of BLM.
  • 序列相似性Belongs to the RMI2 family.
    Contains 1 OB DNA-binding domain.
  • 翻译后修饰Phosphorylated during mitosis.
  • 细胞定位Nucleus. Colocalizes with BLM at nuclear DNA repair foci.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BLAP18 antibody
    • BLM-associated protein of 18 kDa antibody
    • C16orf75 antibody
    • hRMI2 antibody
    • MGC24665 antibody
    • RecQ-mediated genome instability protein 2 antibody
    • RMI2 antibody
    • RMI2_HUMAN antibody
    see all

Anti-RMI2 antibody 图像

  • All lanes : Anti-RMI2 antibody (ab108491) at 1 µg/ml (Milk blocking - 3%)

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 15 kDa
    Observed band size : 17 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 41 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

Anti-RMI2 antibody (ab108491)参考文献

ab108491 has not yet been referenced specifically in any publications.

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