概述

  • 产品名称
  • 描述
    兔多克隆抗体to RMI2
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human RMI2.

  • 阳性对照
    • This antibody gave a positive signal in both HEK293 and MCF7 whole cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab108491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).

靶标

  • 功能
    Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. The complex is therefore essential for the stability, localization, and function of complexes containing BLM. In the RMI complex, it is required to target BLM to chromatin and stress-induced nuclear foci and mitotic phosphorylation of BLM.
  • 序列相似性
    Belongs to the RMI2 family.
    Contains 1 OB DNA-binding domain.
  • 翻译后修饰
    Phosphorylated during mitosis.
  • 细胞定位
    Nucleus. Colocalizes with BLM at nuclear DNA repair foci.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BLAP18 antibody
    • BLM-associated protein of 18 kDa antibody
    • C16orf75 antibody
    • hRMI2 antibody
    • MGC24665 antibody
    • RecQ-mediated genome instability protein 2 antibody
    • RMI2 antibody
    • RMI2_HUMAN antibody
    see all

图片

  • All lanes : Anti-RMI2 antibody (ab108491) at 1 µg/ml (Milk blocking - 3%)

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 15 kDa
    Observed band size: 17 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 41 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes


    The predicted molecular weight of RMI2 is 15 kDa (SwissProt), however we expect to observe a banding pattern around 18 kDa. Abcam recommends using milk as the blocking agent (3%). This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab108491 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

实验方案

文献

ab108491 has not yet been referenced specifically in any publications.

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