概述

  • 产品名称Anti-RLF抗体
    参阅全部 RLF 一抗
  • 描述
    兔多克隆抗体to RLF
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 500-550 of Human RLF (Q13129).

  • 阳性对照
    • HeLa and 293T cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab115154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 218 kDa.
IP Use at 2-10 µg/mg of lysate.

靶标

Anti-RLF antibody 图像

  • All lanes : Anti-RLF antibody (ab115154) at 0.1 µg/ml

    Lane 1 : HeLa cell lysate at 50 µg
    Lane 2 : HeLa cell lysate at 15 µg
    Lane 3 : 293T cell lysate at 50 µg


    Predicted band size : 218 kDa


    Exposure time : 3 minutes
  • Immunoprecipitation of RLF (6 µg of antibody per mg of HeLa cell lysate; 20% of the IP loaded). Lane 1- Immunoprecipitation with an anti-RLF antibody (ab115154); Lane 2- Immunoprecipitation with IgG control. Anti-RLF antibody (ab115154) used at 1 µg/ml for western blot. Detection: Chemiluminescence with exposure time of 10 sec.

Anti-RLF antibody (ab115154)参考文献

ab115154 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"