概述

  • 产品名称Anti-RLF抗体
    参阅全部 RLF 一抗
  • 描述
    兔多克隆抗体to RLF
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Cow, Dog, Pig, Chimpanzee, Macaque Monkey, Gorilla, Chinese Hamster, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1850 to the C-terminus of Human RLF.

  • 阳性对照
    • This antibody gave a positive signal in HeLa whole cell lysate, as well as wild-type Mouse embryo head tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab115011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 280 kDa (predicted molecular weight: 218 kDa).

靶标

Anti-RLF antibody 图像

  • Anti-RLF antibody (ab115011) at 1/500 dilution + Wild-type Mouse Embryo Head tissue at 20 µg

    Secondary
    Anti-Rabbit HRP Conjugated
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 218 kDa
    Observed band size : 280 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 140 kDa (possible cross reactivity).
  • All lanes : Anti-RLF antibody (ab115011) at 1/500 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Anti-Rabbit HRP Conjugated

    Predicted band size : 218 kDa
    Observed band size : 265 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 135 kDa (possible cross reactivity),150 kDa (possible cross reactivity).

Anti-RLF antibody (ab115011)参考文献

This product has been referenced in:
  • Daxinger L  et al. An ENU mutagenesis screen identifies novel and known genes involved in epigenetic processes in the mouse. Genome Biol 14:R96 (2013). Read more (PubMed: 24025402) »

See 1 Publication for this product

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