Anti-RIZ1抗体[33AT1045] (ab3791)


  • 产品名称Anti-RIZ1抗体[33AT1045]
    参阅全部 RIZ1 一抗
  • 描述
    小鼠单克隆抗体[33AT1045] to RIZ1
  • 特异性This antibody is specific for RIZ 1. It does not recognize the beta isoform (RIZ 2).
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Fusion protein, corresponding to amino acids 1-347 of Human RIZ1

  • 常规说明

    Similar to acetylation and phosphorylation, histone methylation at the N-terminal tail has emerged as an important role in regulating chromatin dynamics and gene activity. Histone methylation occurs on arginine and lysine residues and is catalyzed by two families of proteins, the protein arginine methyltransferase family and the SET-domain-containing methyltransferase family. Five members have been identified in the arginine methyltransferase family. About 27 are grouped into the SET-domain family, and another 17 make up the PR domain family that is related to the SET domain family. The retinoblastoma protein-interacting zinc finger gene RIZ 1 is a tumor suppressor gene and a FOUNDING member of the PR domain family. RIZ 1 inactivation is commonly found in many types of human cancers and occurs through loss of mRNA expression, frame shift mutation, chromosomal deletion, and missense mutation. RIZ 1 is also a tumor susceptibility gene in mice. The loss of RIZ 1 mRNA in human cancers was shown to associate with DNA methylation of its promoter CpG island. Methylation of the RIZ 1 promoter strongly correlated with lost or decreased RIZ 1 mRNA expression in breast, liver, colon, and lung cancer cell lines as well as in liver cancer tissues.



Our Abpromise guarantee covers the use of ab3791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 189 kDa.
ELISA 1/1000.


  • 功能S-adenosyl-L-methionine-dependent histone methyltransferase that specifically methylates 'Lys-9' of histone H3. May function as a DNA-binding transcription factor. Binds to the macrophage-specific TPA-responsive element (MTE) of the HMOX1 (heme oxygenase 1) gene and may act as a transcriptional activator of this gene.
  • 组织特异性Highly expressed in retinoblastoma cell lines and in brain tumors. Also expressed in a number of other cell lines and in brain, heart, skeletal muscle, liver and spleen. Isoform 1 is expressed in testis at much higher level than isoform 3.
  • 序列相似性Contains 8 C2H2-type zinc fingers.
    Contains 1 SET domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GATA-3-binding protein G3B antibody
    • HUMHOXY1 antibody
    • KMT8 antibody
    • Lysine N-methyltransferase 8 antibody
    • MTB-ZF antibody
    • MTE-binding protein antibody
    • PR domain containing 2, with ZNF domain antibody
    • PR domain zinc finger protein 2 antibody
    • PR domain-containing protein 2 antibody
    • PRDM2 antibody
    • PRDM2_HUMAN antibody
    • Retinoblastoma protein binding zinc finger protein antibody
    • Retinoblastoma protein-interacting zinc finger protein antibody
    • Retinoblastoma protein-interacting zinc-finger protein antibody
    • RIZ antibody
    • RIZ1 antibody
    • RIZ2 antibody
    • Zinc finger DNA binding protein antibody
    • Zinc finger protein RIZ antibody
    see all

Anti-RIZ1 antibody [33AT1045] 图像

  • Predicted band size : 189 kDa

    Western blot analysis of ab3791 in lysate from transformed cells. Lane A: mock plasmid, Lane B: RIZ1[1-347]-encoding plasmid. RIZ1[1-347] (arrow) was detected using ab3791. Secondary HRP-anti-mouse was used for signal visualization with chemiluminescence.

Anti-RIZ1 antibody [33AT1045] (ab3791)参考文献

This product has been referenced in:
  • Jiang GL & Huang S Adenovirus expressing RIZ1 in tumor suppressor gene therapy of microsatellite-unstable colorectal cancers. Cancer Res 61:1796-8 (2001). Read more (PubMed: 11280725) »
  • Liu L  et al. The retinoblastoma interacting zinc finger gene RIZ produces a PR domain-lacking product through an internal promoter. J Biol Chem 272:2984-91 (1997). Read more (PubMed: 9006946) »

See all 2 Publications for this product

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Thank you for submitting an Abreview of ab3791. As you may have noticed, your review has now been published on our website.
Since you obtained poor results using the antibody in a tested species and application, we would like to follow up on this ...

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Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse ES Cells)
Loading amount 60 µg
Specification Mouse ES Cells
Gel Running Conditions Reduced Denaturing (6%)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Mr. Utkarsh Kapoor

Verified customer

提交于 Dec 19 2012