Anti-RIT1抗体(ab53720)

概述

  • 产品名称Anti-RIT1抗体
    参阅全部 RIT1 一抗
  • 描述
    兔多克隆抗体to RIT1
  • 经测试应用适用于: ICC/IF, ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide (Human)

  • 阳性对照
    • Jurkat cells

性能

应用

Our Abpromise guarantee covers the use of ab53720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
ELISA 1/10000.
WB 1/500 - 1/1000. Predicted molecular weight: 25 kDa.

靶标

  • 功能Plays a crucial role in coupling NGF stimulation to the activation of both EPHB2 and MAPK14 signaling pathways and in NGF-dependent neuronal differentiation.
  • 组织特异性Expressed in many tissues.
  • 序列相似性Belongs to the small GTPase superfamily. Ras family.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GTP binding protein Roc1 antibody
    • GTP-binding protein Rit1 antibody
    • NS8 antibody
    • Ras like protein expressed in many tissues antibody
    • Ras like without CAAX 1 antibody
    • Ras-like protein expressed in many tissues antibody
    • Ras-like without CAAX protein 1 antibody
    • RIBB antibody
    • Ric like expressed in many tissues antibody
    • Ric-like protein without CAAX motif 1 antibody
    • RIT antibody
    • RIT1 antibody
    • RIT1_HUMAN antibody
    • ROC1 antibody
    see all

Anti-RIT1 antibody 图像

  • ICC/IF image of ab53720 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53720, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-RIT1 antibody (ab53720)参考文献

ab53720 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"