Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
Synthetic peptide within Human RIPX aa 414-463 (C terminal). The exact sequence is proprietary.
Database link: Q7L099
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 53 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Implicated in the formation of a single axon by developing neurons. May inhibit the formation of additional axons by inhibition of PI3K in minor neuronal processes.
Contains 1 RUN domain.
Isoform 1 is partially phosphorylated.
Cell projection. Enriched in F-actin enriched filopodia and lamellipodia of growth cones of axons and minor processes of hippocampal neurons in culture.
Information by UniProt
- Protein RUFY3 antibody
- rap2 interacting protein x antibody
- Rap2 interacting protein x isoform 2 antibody
Western blot - Anti-RIPX antibody (ab30986)
Anti-RIPX antibody (ab30986) at 1 µg/ml (in 5% skim milk / PBS buffer) + Jurkay cell lysateSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size:
53 kDaObserved band size:
58 kDa (why is the actual band size different from the predicted?
Gel concentration: 12%.
has not yet been referenced specifically in any publications.
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