Anti-RIP3 (phospho T231 + S232)抗体[2D7] (ab205421)
Key features and details
- Mouse monoclonal [2D7] to RIP3 (phospho T231 + S232)
- Suitable for: ELISA, WB
- Reacts with: Mouse
- Isotype: IgG1
概述
-
产品名称
Anti-RIP3 (phospho T231 + S232)抗体[2D7]
参阅全部 RIP3 一抗 -
描述
小鼠单克隆抗体[2D7] to RIP3 (phospho T231 + S232) -
宿主
Mouse -
经测试应用
适用于: ELISA, WBmore details -
种属反应性
与反应: Mouse -
免疫原
Synthetic peptide corresponding to Mouse RIP3 (phospho T231 + S232).
-
阳性对照
- WB: Mouse pancreas, kidney and small intestine tissue lysate.
-
常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
-
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
2D7 -
同种型
IgG1 -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab205421于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ELISA |
Use at an assay dependent concentration.
|
|
WB |
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
|
说明 |
---|
ELISA
Use at an assay dependent concentration. |
WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa). |
靶标
-
功能
Promotes apoptosis. -
组织特异性
Detected at lower levels in heart, placenta, lung and kidney. Isoform 3 is significantly increased in colon and lung cancers. -
序列相似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated. -
细胞定位
Cytoplasm. - Information by UniProt
-
数据库链接
- Entrez Gene: 56532 Mouse
- SwissProt: Q9QZL0 Mouse
- Unigene: 46612 Mouse
-
别名
- Receptor interacting protein 3 antibody
- Receptor interacting serine threonine kinase 3 antibody
- Receptor interacting serine/threonine protein kinase 3 antibody
see all
图片
-
All lanes : Anti-RIP3 (phospho T231 + S232) antibody [2D7] (ab205421) at 1 µg/ml
Lane 1 : Mouse Pancreas Tissue Lysate at 10 µg
Lane 2 : Mouse Kidney Tissue Lysate at 20 µg
Lane 3 : Mouse Small Intestine Tissue Lysate at 20 µg
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa
Exposure time: 4 minutesBlocking buffer: 2% BSA.
Performed under reducing conditions.
-
Serially diluted ab205421 was bound to immobilised RIP3 phospho peptide (T231), RIP3 phospho peptide (S232), RIP3 dual phospho peptide (T231+S232) or RIP3 control peptide (RIP3-nP; all peptides at 1 microgram x mL-1). The antibody was detected by HRP-labeled goat anti-mouse IgG and signal was developed with TMB substrate.
数据表及文件
-
Datasheet download
文献 (14)
ab205421 被引用在 14 文献中.
- Zhang R et al. Bclaf1 regulates c-FLIP expression and protects cells from TNF-induced apoptosis and tissue injury. EMBO Rep 23:e52702 (2022). PubMed: 34693625
- Chen XC et al. Metformin improves renal injury of MRL/lpr lupus-prone mice via the AMPK/STAT3 pathway. Lupus Sci Med 9:N/A (2022). PubMed: 35414608
- Li Y et al. Generative deep learning enables the discovery of a potent and selective RIPK1 inhibitor. Nat Commun 13:6891 (2022). PubMed: 36371441
- Ye L et al. Lansoprazole promotes cisplatin-induced acute kidney injury via enhancing tubular necroptosis. J Cell Mol Med 25:2703-2713 (2021). PubMed: 33605079
- Zhang Y et al. A unique death pathway keeps RIPK1 D325A mutant mice in check at embryonic day 10.5. PLoS Biol 19:e3001304 (2021). PubMed: 34437534