Anti-RIP3抗体[Rippy-3] (ab16090)

概述

  • 产品名称Anti-RIP3抗体[Rippy-3]
    参阅全部 RIP3 一抗
  • 描述
    小鼠单克隆抗体[Rippy-3] to RIP3
  • 特异性The antibody has been tested on HEK 293T cells transfected with an expression plasmid coding for human RIP3. It has not been tested against the endogenous protein.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant full length protein corresponding to Human RIP3.
    Database link: Q9Y573

  • 阳性对照
    • recombinant protein

性能

应用

Our Abpromise guarantee covers the use of ab16090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 62 kDa.

靶标

  • 功能Promotes apoptosis.
  • 组织特异性Detected at lower levels in heart, placenta, lung and kidney. Isoform 3 is significantly increased in colon and lung cancers.
  • 序列相似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • 翻译后修饰Autophosphorylated.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Receptor interacting protein 3 antibody
    • Receptor interacting serine threonine kinase 3 antibody
    • Receptor interacting serine/threonine protein kinase 3 antibody
    • Receptor-interacting protein 3 antibody
    • Receptor-interacting serine/threonine-protein kinase 3 antibody
    • RIP 3 antibody
    • RIP like protein kinase 3 antibody
    • RIP-3 antibody
    • RIP-like protein kinase 3 antibody
    • RIPK 3 antibody
    • RIPK3 antibody
    • RIPK3_HUMAN antibody
    see all

Anti-RIP3 antibody [Rippy-3] 图像

  • All lanes : Anti-RIP3 antibody [Rippy-3] (ab16090) at 1/1000 dilution

    Lane 1 : HEK 293T cells(5x105) mock transfected
    Lane 2 : HEK 293T cells (5x105)transfected with an expression plasmid coding for human RIP3


    Predicted band size : 62 kDa

Anti-RIP3 antibody [Rippy-3] (ab16090)参考文献

ab16090 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. We do have positive controls available for these antibodies. The Jurkat lysate catalog number is ab7899. This should also be an appropriate control for the anti-RIP3 antibody, as we do not have the HEK293 transfected cells ...

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Merci pour votre patience.

Notre recherche interne donnais comme seul candidat l'anticorpsab72106 qui pourrait avoir été utilisé dans la publication que vous nous avez cité. Malheureusement est il très peu p...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (MEF cells)
Loading amount 15 µg
Specification MEF cells
Treatment hydroxyurea for 1h
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Mar 26 2007

Thank you for providing the customer's results and protocol, it is very clear that there is a problem with the antibody and I will investigate this matter further and will consider removing this antibody from our catalogue. I have asked our accounts...

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Thank you for providing so much information about the Western Blotting protocol used with this antibody. The researcher has clearly tried many steps to optimise the experimental conditions, but there's one thing they have not yet tried which I think wi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"