概述

  • 产品名称
  • 描述
    兔多克隆抗体to RIP3
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human, Chimpanzee
  • 免疫原

    Synthetic peptide within Human RIP3 aa 480-510. The exact sequence is proprietary.
    Database link: Q9Y578
    (Peptide available as ab204450)

  • 阳性对照
    • NIH3T3 cell lysate; Paraffin embedded human pancreatic tissue

性能

应用

Our Abpromise guarantee covers the use of ab72106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
IHC-P Use a concentration of 10 µg/ml.

靶标

  • 功能
    Promotes apoptosis.
  • 组织特异性
    Detected at lower levels in heart, placenta, lung and kidney. Isoform 3 is significantly increased in colon and lung cancers.
  • 序列相似性
    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • 翻译后修饰
    Autophosphorylated.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Receptor interacting protein 3 antibody
    • Receptor interacting serine threonine kinase 3 antibody
    • Receptor interacting serine/threonine protein kinase 3 antibody
    • Receptor-interacting protein 3 antibody
    • Receptor-interacting serine/threonine-protein kinase 3 antibody
    • RIP 3 antibody
    • RIP like protein kinase 3 antibody
    • RIP-3 antibody
    • RIP-like protein kinase 3 antibody
    • RIPK 3 antibody
    • RIPK3 antibody
    • RIPK3_HUMAN antibody
    see all

Anti-RIP3 antibody 图像

  • Immunohistochemical analysis of RIP3 expression in paraffin embedded human pancreatic tissue using ab72106 at 10ug/ml.

Anti-RIP3 antibody (ab72106)参考文献

This product has been referenced in:
  • Zhou X  et al. The bile acid receptor FXR attenuates acinar cell autophagy in chronic pancreatitis. Cell Death Discov 3:17027 (2017). WB ; Human . Read more (PubMed: 28660075) »
  • Zhang Y  et al. RIP1 autophosphorylation is promoted by mitochondrial ROS and is essential for RIP3 recruitment into necrosome. Nat Commun 8:14329 (2017). Read more (PubMed: 28176780) »

See all 7 Publications for this product

Product Wall

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Human Cell lysate - whole cell (HT-29 Human adenocarcinoma)
Specification
HT-29 Human adenocarcinoma
Treatment
48 hrs, DMEM no serum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1µg/mL · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Feb 05 2014

Merci pour votre patience.

Notre recherche interne donnais comme seul candidat l'anticorpsab72106 qui pourrait avoir été utilisé dans la publication que vous nous avez cité. Malheureusement est il très peu p...

Read More

Thank you for kindly confirming these details as this enables us to closely monitor the quality of our products

We would be pleased to arrange a replacement product free of charge. I am forwarding these details on to our Distributor’s ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册