概述

  • 产品名称Anti-RIP3抗体
    参阅全部 RIP3 一抗
  • 描述
    兔多克隆抗体to RIP3
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human, Chimpanzee
  • 免疫原

    Synthetic peptide within Human RIP3 aa 480-510. The exact sequence is proprietary.
    Database link: Q9Y578
    (Peptide available as ab204450)

  • 阳性对照
    • NIH3T3 cell lysate; Paraffin embedded human pancreatic tissue

性能

应用

Our Abpromise guarantee covers the use of ab72106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
IHC-P Use a concentration of 10 µg/ml.

靶标

  • 功能Promotes apoptosis.
  • 组织特异性Detected at lower levels in heart, placenta, lung and kidney. Isoform 3 is significantly increased in colon and lung cancers.
  • 序列相似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • 翻译后修饰Autophosphorylated.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Receptor interacting protein 3 antibody
    • Receptor interacting serine threonine kinase 3 antibody
    • Receptor interacting serine/threonine protein kinase 3 antibody
    • Receptor-interacting protein 3 antibody
    • Receptor-interacting serine/threonine-protein kinase 3 antibody
    • RIP 3 antibody
    • RIP like protein kinase 3 antibody
    • RIP-3 antibody
    • RIP-like protein kinase 3 antibody
    • RIPK 3 antibody
    • RIPK3 antibody
    • RIPK3_HUMAN antibody
    see all

Anti-RIP3 antibody 图像

  • Immunohistochemical analysis of RIP3 expression in paraffin embedded human pancreatic tissue using ab72106 at 10ug/ml.

Anti-RIP3 antibody (ab72106)参考文献

This product has been referenced in:
  • Omoto S  et al. Suppression of RIP3-dependent necroptosis by human cytomegalovirus. J Biol Chem 290:11635-48 (2015). Read more (PubMed: 25778401) »
  • Zhao J  et al. Mixed lineage kinase domain-like is a key receptor interacting protein 3 downstream component of TNF-induced necrosis. Proc Natl Acad Sci U S A 109:5322-7 (2012). WB ; Human . Read more (PubMed: 22421439) »

See all 3 Publications for this product

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Human Cell lysate - whole cell (HT-29 Human adenocarcinoma)
Specification HT-29 Human adenocarcinoma
Treatment 48 hrs, DMEM no serum
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1µg/mL · Temperature: 22°C
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提交于 Feb 05 2014

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"