概述

  • 产品名称Anti-RIP3抗体
    参阅全部 RIP3 一抗
  • 描述
    兔多克隆抗体to RIP3
  • 经测试应用适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Rat RIP3 (C terminal).
    Database link: Q9Z2P5

  • 阳性对照
    • BALB/3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate IF/ICC: SV40LT-SMC cell line

性能

应用

Our Abpromise guarantee covers the use of ab62344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).

Blocking peptide available ab8464

ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml.

靶标

  • 功能Promotes apoptosis.
  • 组织特异性Detected at lower levels in heart, placenta, lung and kidney. Isoform 3 is significantly increased in colon and lung cancers.
  • 序列相似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • 翻译后修饰Autophosphorylated.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Receptor interacting protein 3 antibody
    • Receptor interacting serine threonine kinase 3 antibody
    • Receptor interacting serine/threonine protein kinase 3 antibody
    • Receptor-interacting protein 3 antibody
    • Receptor-interacting serine/threonine-protein kinase 3 antibody
    • RIP 3 antibody
    • RIP like protein kinase 3 antibody
    • RIP-3 antibody
    • RIP-like protein kinase 3 antibody
    • RIPK 3 antibody
    • RIPK3 antibody
    • RIPK3_HUMAN antibody
    see all

Anti-RIP3 antibody 图像

  • All lanes : Anti-RIP3 antibody (ab62344) at 1 µg/ml

    Lane 1 : Whole cell lysate in the absence of RIP3 peptide
    Lane 2 : Whole cell lysate in the presence of RIP3 peptide


    Predicted band size : 57 kDa
    Observed band size : 57 kDa
  • ab62344 at 5 µg/ml staining rat kidney tissue by IHC-P.
  • Immunofluorescence of RIP3 in Rat Kidney cells using ab62344 at 20 ug/ml.

  • ICC/IF image of ab62344 stained SV40LT-SMC cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62344, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-RIP3 antibody (ab62344)参考文献

This product has been referenced in:
  • Furr SR  et al. A role for DNA-dependent activator of interferon regulatory factor in the recognition of herpes simplex virus type 1 by glial cells. J Neuroinflammation 8:99 (2011). WB ; Mouse . Read more (PubMed: 21838860) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab62344.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"