概述

  • 产品名称Anti-RIP3抗体
    参阅全部 RIP3 一抗
  • 描述
    兔多克隆抗体to RIP3
  • 经测试应用适用于: IP, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Horse, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to Human RIP3 aa 168-217. Immunogen is located within the provided sequence.
    Sequence:

    GGSQSGTGSGEPGGTLGYLAPELFVNVNRKASTASDVYSFGILMWAVLAG


    Database link: Q9Y572
    (Peptide available as ab178834)

  • 阳性对照
    • Jurkat cell lysate (Western blot). Human pancreas (IHC-P).

性能

应用

Our Abpromise guarantee covers the use of ab56164 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).Can be blocked with Human RIP3 peptide (ab178834). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 3 - 12 µg/ml.

靶标

  • 功能Promotes apoptosis.
  • 组织特异性Detected at lower levels in heart, placenta, lung and kidney. Isoform 3 is significantly increased in colon and lung cancers.
  • 序列相似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • 翻译后修饰Autophosphorylated.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Receptor interacting protein 3 antibody
    • Receptor interacting serine threonine kinase 3 antibody
    • Receptor interacting serine/threonine protein kinase 3 antibody
    • Receptor-interacting protein 3 antibody
    • Receptor-interacting serine/threonine-protein kinase 3 antibody
    • RIP 3 antibody
    • RIP like protein kinase 3 antibody
    • RIP-3 antibody
    • RIP-like protein kinase 3 antibody
    • RIPK 3 antibody
    • RIPK3 antibody
    • RIPK3_HUMAN antibody
    see all

Anti-RIP3 antibody 图像

  • All lanes : Anti-RIP3 antibody (ab56164) at 1/1000 dilution

    Lane 1 : mouse small intestine
    Lane 2 : mouse liver

    Lysates/proteins at 20 µg per lane.

    Secondary
    goat anti-rabbit secondary antibody at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 57 kDa
    Additional bands at : 57 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 40 seconds

    This image is courtesy of an anonymous Abreview

    Blocked with 5% milk for 1 hour at 25C

    See Abreview

  • Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded human pancreas sections labelling RIP3 with ab56164 at 3 - 12 µg/mL. The secondary antibody used was a donkey anti-rabbit antibody at 1/200. The exposure time was 0.2 seconds at magnification of 20X. 

  • RIP3 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to RIP3 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab56164.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 65kDa; RIP3.
  • All lanes : Anti-RIP3 antibody (ab56164) at 1.25 µg/ml

    Lane 1 : Molecular weight markers
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 57 kDa
    Observed band size : 57 kDa
    Gel concentration: 12%

Anti-RIP3 antibody (ab56164)参考文献

This product has been referenced in:
  • Gautheron J  et al. The necroptosis-inducing kinase RIPK3 dampens adipose tissue inflammation and glucose intolerance. Nat Commun 7:11869 (2016). WB ; Mouse . Read more (PubMed: 27323669) »
  • Karch J  et al. Necroptosis Interfaces with MOMP and the MPTP in Mediating Cell Death. PLoS One 10:e0130520 (2015). Read more (PubMed: 26061004) »

See all 8 Publications for this product

Product Wall

Application Western blot
Sample Rat Cell lysate - whole cell (H9C2 (myoblasts))
Gel Running Conditions Reduced Denaturing (12% gel)
Loading amount 20 µg
Specification H9C2 (myoblasts)
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提交于 Nov 18 2016

Application Immunoprecipitation
Sample Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts (MEFs))
Total protein in input 1e+007 cells
Immuno-precipitation step Other - Protein G Dynabeads
Specification Mouse embryonic fibroblasts (MEFs)
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提交于 Oct 27 2016

Application Immunoprecipitation
Sample Human Cell lysate - whole cell (A549 (human lung epithelium cells))
Total protein in input 5e+006 cells
Treatment 100 units/mL human IFNbeta
Immuno-precipitation step Protein G
Specification A549 (human lung epithelium cells)
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提交于 Oct 27 2016

Application Immunoprecipitation
Sample Dog Cell lysate - whole cell (Canine Kidney Epithelial MDCK cells)
Total protein in input 5e+006 cells
Immuno-precipitation step Other - Protein G Dynabeads
Specification Canine Kidney Epithelial MDCK cells
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提交于 Oct 25 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (jurkat)
Permeabilization Yes - perm buffer by biolegend
Gating Strategy live cells according to fixable Zombie dye
Specification jurkat
Preparation Cell harvesting/tissue preparation method: fix and perm with biolegend buffer set
Sample buffer: perm buffer by biolegend
Fixation Paraformaldehyde
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提交于 Oct 18 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Dog Cell lysate - whole cell (Canine Kidney Epithelial MDCK cells)
Gel Running Conditions Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)
Loading amount 500000 cells
Specification Canine Kidney Epithelial MDCK cells
Blocking step Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
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提交于 Sep 27 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (A549 (human lung epithelium cells))
Gel Running Conditions Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)
Loading amount 50000 cells
Specification A549 (human lung epithelium cells)
Blocking step Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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提交于 Sep 01 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts (MEFs))
Gel Running Conditions Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)
Loading amount 30000 cells
Specification Mouse embryonic fibroblasts (MEFs)
Blocking step Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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提交于 Aug 31 2016

Application Western blot
Sample Hamster Cell lysate - whole cell (BHK-21 (Kidney Fibroblasts))
Gel Running Conditions Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)
Loading amount 1e+006 cells
Specification BHK-21 (Kidney Fibroblasts)
Blocking step Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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提交于 Aug 31 2016

Application Western blot
Sample African Green Monkey Cell lysate - whole cell (VERO E6)
Gel Running Conditions Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)
Loading amount 50000 cells
Specification VERO E6
Blocking step Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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提交于 Aug 31 2016

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