Anti-RIP3抗体(ab226297)
Key features and details
- Rabbit polyclonal to RIP3
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RIP3抗体
参阅全部 RIP3 一抗 -
描述
兔多克隆抗体to RIP3 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Gorilla, Orangutan -
免疫原
Synthetic peptide within Human RIP3 aa 400-450. The exact sequence is proprietary. (NP_006862.2).
Database link: Q9Y572 -
阳性对照
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab226297 was affinity purified using an epitope specific to RIP3 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab226297于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 57 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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说明 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 57 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
Promotes apoptosis. -
组织特异性
Detected at lower levels in heart, placenta, lung and kidney. Isoform 3 is significantly increased in colon and lung cancers. -
序列相似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 452820 Chimpanzee
- Entrez Gene: 101126247 Gorilla
- Entrez Gene: 11035 Human
- Entrez Gene: 100461174 Orangutan
- Omim: 605817 Human
- SwissProt: Q9Y572 Human
- Unigene: 268551 Human
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别名
- Receptor interacting protein 3 antibody
- Receptor interacting serine threonine kinase 3 antibody
- Receptor interacting serine/threonine protein kinase 3 antibody
see all
图片
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All lanes : Anti-RIP3 antibody (ab226297) at 0.4 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate prepared using NETN buffer
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate prepared using NETN buffer
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate prepared using NETN buffer
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate prepared using RIPA buffer
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 57 kDa
Exposure time: 30 seconds -
RIP3 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (prepared using RIPA buffer; 1 mg for IP, 20% of IP loaded) with ab226297 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226297 at 1 µg/ml.
Lane 1: ab226297 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab226297 被引用在 1 文献中.
- Guo R et al. Activation of Delta-Opioid Receptor Protects ARPE19 Cells against Oxygen-Glucose Deprivation/Reoxygenation-Induced Necroptosis and Apoptosis by Inhibiting the Release of TNF-α. J Ophthalmol 2022:2285663 (2022). PubMed: 36457949