概述

  • 产品名称
  • 描述
    兔多克隆抗体to RIP2
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide:

    PTIPYHKLADLRYLSRGASG

    , corresponding to amino acids 11/30 of Human RIP2. (Peptide available as ab8463.)

  • 阳性对照
    • A431 or K562 whole cell lysate An approximately60 kDa band can be detected.

应用

Our Abpromise guarantee covers the use of ab8428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 60 kDa.Can be blocked with Human RIP2 peptide (ab8463).
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能
    Activates pro-caspase-1 and pro-caspase-8. Potentiates CASP8-mediated apoptosis. Activates NF-kappa-B.
  • 组织特异性
    Detected in heart, brain, placenta, lung, peripheral blood leukocytes, spleen, kidney, testis, prostate, pancreas and lymph node.
  • 序列相似性
    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 1 CARD domain.
    Contains 1 protein kinase domain.
  • 翻译后修饰
    Autophosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination catalyzed by ITCH.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CARD 3 antibody
    • CARD carrying kinase antibody
    • CARD containing ICE associated kinase antibody
    • CARD containing IL 1 beta ICE kinase antibody
    • CARD containing IL1 beta ICE kinase antibody
    • CARD containing interleukin 1 beta converting enzyme (ICE) associated kinase antibody
    • CARD containing interleukin 1 beta converting enzyme associated kinase antibody
    • CARD-containing IL-1 beta ICE-kinase antibody
    • CARD-containing interleukin-1 beta-converting enzyme-associated kinase antibody
    • CARD3 antibody
    • CARDIAK antibody
    • CCK antibody
    • CLARP kinase antibody
    • GIG 30 antibody
    • GIG30 antibody
    • Growth inhibiting gene 30 antibody
    • Receptor interacting protein (RIP) like interacting caspase like apoptosis regulatory protein (CLARP) kinase antibody
    • Receptor interacting protein 2 antibody
    • Receptor interacting serine threonine kinase 2 antibody
    • Receptor interacting serine/threonine protein kinase 2 antibody
    • Receptor-interacting protein 2 antibody
    • Receptor-interacting serine/threonine-protein kinase 2 antibody
    • RICK antibody
    • RIP 2 antibody
    • RIP like interacting CLARP kinase antibody
    • RIP-2 antibody
    • RIP-like-interacting CLARP kinase antibody
    • RIPK 2 antibody
    • Ripk2 antibody
    • RIPK2_HUMAN antibody
    • TNFRSF antibody
    • Tyrosine-protein kinase RIPK2 antibody
    • UNQ277/PRO314/PRO34092 antibody
    see all

Anti-RIP2 antibody 图像

  • Western Blot of HeLa (1), Ramos (2) and EL4 (3) cell lysates labeling RIP2 with Anti-RIP2 antibody (ab8428) at 1µg/ml.
  • All lanes : Anti-RIP2 antibody (ab8428) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : K562 whole cell lysate


    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • ab8428 at 10µg/ml staining RIP2 in A431 cells by ICC/IF 

Anti-RIP2 antibody (ab8428)参考文献

This product has been referenced in:
  • Emmerich CH  et al. Lys63/Met1-hybrid ubiquitin chains are commonly formed during the activation of innate immune signalling. Biochem Biophys Res Commun 474:452-461 (2016). Read more (PubMed: 27133719) »
  • Hollenbach E  et al. Inhibition of RICK/nuclear factor-kappaB and p38 signaling attenuates the inflammatory response in a murine model of Crohn disease. J Biol Chem 280:14981-8 (2005). Read more (PubMed: 15691843) »

See all 2 Publications for this product

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