Anti-RIP140抗体(ab42126)
Key features and details
- Rabbit polyclonal to RIP140
- Suitable for: IP, WB, IHC-Fr, IHC (PFA fixed), ChIP, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-RIP140抗体
参阅全部 RIP140 一抗 -
描述
兔多克隆抗体to RIP140 -
宿主
Rabbit -
特异性
ab42126 recognises the C terminal domain of RIP140 -
经测试应用
适用于: IP, WB, IHC-Fr, IHC (PFA fixed), ChIP, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide (Human) corresponding to the C terminal domain of RIP140. Peptide available as ab93493.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Constituents: 0.5% BSA, 30% Glycerol (glycerin, glycerine), 69.5% Tris HCl -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab42126于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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IHC (PFA fixed) |
Use at an assay dependent concentration.
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ChIP |
Use at an assay dependent concentration. PubMed: 21360626
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IHC-P | (1) |
Use a concentration of 4 µg/ml.
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ICC/IF |
Use a concentration of 5 µg/ml.
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说明 |
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IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
IHC (PFA fixed)
Use at an assay dependent concentration. |
ChIP
Use at an assay dependent concentration. PubMed: 21360626 |
IHC-P
Use a concentration of 4 µg/ml. |
ICC/IF
Use a concentration of 5 µg/ml. |
靶标
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功能
Modulates transcriptional activation by steroid receptors such as NR3C1, NR3C2 and ESR1. Also modulates transcriptional repression by nuclear hormone receptors. -
结构域
Contains 9 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs, which have different affinities for nuclear receptors. The C-terminal LTKTNPILYYMLQK motif is required for ligand-dependent interaction with RAAR and RXRB homodimers and heterodimers, for the corepressor activity, and for the formation of an HDAC3 complex with RARA/RXRB (By similarity). Contains at least four autonomous repression domains (RD1-4). RD1 functions via a histone deacetylase (HDAC)-independent mechanism, whereas RD2, RD3 and RD4 can function by HDAC-dependent or independent mechanisms, depending on cell type. RD2 is dependent on CTBP binding. -
翻译后修饰
Acetylation regulates its nuclear translocation and corepressive activity (By similarity). Acetylation abolishes interaction with CTBP1. Phosphorylation enhances interaction with YWHAH. -
细胞定位
Nucleus. Localized to discrete foci and redistributes to larger nuclear domains upon binding to ligand-bound NR3C1. - Information by UniProt
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数据库链接
- Entrez Gene: 8204 Human
- Entrez Gene: 268903 Mouse
- Entrez Gene: 304157 Rat
- Omim: 602490 Human
- SwissProt: P48552 Human
- SwissProt: Q8CBD1 Mouse
- Unigene: 155017 Human
- Unigene: 455873 Mouse
see all -
别名
- NRIP 1 antibody
- NRIP1 antibody
- NRIP1_HUMAN antibody
see all
图片
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ab42126 (4µg/ml) staining RIP140 in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is staining of the nuclei of the intestinal glands.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
ICC/IF image of ab42126 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42126, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
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Datasheet download
文献 (35)
ab42126 被引用在 35 文献中.
- Jacquier V et al. RIP140 inhibits glycolysis-dependent proliferation of breast cancer cells by regulating GLUT3 expression through transcriptional crosstalk between hypoxia induced factor and p53. Cell Mol Life Sci 79:270 (2022). PubMed: 35501580
- Lin YW et al. Regulation of exosome secretion by cellular retinoic acid binding protein 1 contributes to systemic anti-inflammation. Cell Commun Signal 19:69 (2021). PubMed: 34193153
- Gleizes A et al. RIP140 Represses Intestinal Paneth Cell Differentiation and Interplays with SOX9 Signaling in Colorectal Cancer. Cancers (Basel) 13:N/A (2021). PubMed: 34206767
- Palassin P et al. A Truncated NRIP1 Mutant Amplifies Microsatellite Instability of Colorectal Cancer by Regulating MSH2/MSH6 Expression, and Is a Prognostic Marker of Stage III Tumors. Cancers (Basel) 13:N/A (2021). PubMed: 34503257
- Tsagkaraki E et al. CRISPR-enhanced human adipocyte browning as cell therapy for metabolic disease. Nat Commun 12:6931 (2021). PubMed: 34836963