概述

  • 产品名称
  • 描述
    兔多克隆抗体to RIP140
  • 特异性
    ab42125 recognises RIP140 at the N terminal region containing the HDAC binding domain.
  • 经测试应用
    适用于: IP, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (Human) corresponding to the N terminal domain of RIP140.

性能

应用

Our Abpromise guarantee covers the use of ab42125 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP 1/200.
WB 1/1000. Predicted molecular weight: 129 kDa.

靶标

  • 功能
    Modulates transcriptional activation by steroid receptors such as NR3C1, NR3C2 and ESR1. Also modulates transcriptional repression by nuclear hormone receptors.
  • 结构域
    Contains 9 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs, which have different affinities for nuclear receptors. The C-terminal LTKTNPILYYMLQK motif is required for ligand-dependent interaction with RAAR and RXRB homodimers and heterodimers, for the corepressor activity, and for the formation of an HDAC3 complex with RARA/RXRB (By similarity). Contains at least four autonomous repression domains (RD1-4). RD1 functions via a histone deacetylase (HDAC)-independent mechanism, whereas RD2, RD3 and RD4 can function by HDAC-dependent or independent mechanisms, depending on cell type. RD2 is dependent on CTBP binding.
  • 翻译后修饰
    Acetylation regulates its nuclear translocation and corepressive activity (By similarity). Acetylation abolishes interaction with CTBP1. Phosphorylation enhances interaction with YWHAH.
  • 细胞定位
    Nucleus. Localized to discrete foci and redistributes to larger nuclear domains upon binding to ligand-bound NR3C1.
  • Information by UniProt
  • 数据库链接
  • 别名
    • NRIP 1 antibody
    • NRIP1 antibody
    • NRIP1_HUMAN antibody
    • Nuclear factor RIP 140 antibody
    • Nuclear factor RIP140 antibody
    • Nuclear receptor interacting protein 1 antibody
    • Nuclear receptor-interacting protein 1 antibody
    • Receptor interacting protein 140 antibody
    • Receptor-interacting protein 140 antibody
    • RIP 140 antibody
    • RIP140 antibody
    see all

文献

This product has been referenced in:
  • Zou R  et al. Involvement of receptor-interacting protein 140 in palmitate-stimulated macrophage infiltration of pancreatic beta cells. Exp Ther Med 14:483-494 (2017). Read more (PubMed: 28672957) »
  • Ding Z  et al. Spy1 induces de-ubiquitinating of RIP1 arrest and confers glioblastoma's resistance to tumor necrosis factor (TNF-a)-induced apoptosis through suppressing the association of CLIPR-59 and CYLD. Cell Cycle 14:2149-59 (2015). Read more (PubMed: 26017671) »

See all 3 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - nuclear (MCF7)
Loading amount
50 µg
Specification
MCF7
Gel Running Conditions
Non-reduced Denaturing (8% Acrylamide)
Blocking step
Milk as blocking agent for 25 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 01 2011

Application
Western blot
Sample
Monkey Cell lysate - nuclear (COS7)
Loading amount
50 µg
Specification
COS7
Gel Running Conditions
Non-reduced Denaturing (6% Acrylamide)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Nov 30 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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