Anti-RIP抗体(ab2035)

概述

  • 产品名称Anti-RIP抗体
    参阅全部 RIP 一抗
  • 描述
    兔多克隆抗体to RIP
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cow
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region between amino acids 420-433 of Human RIPK1.

  • 阳性对照
    • Human muscle lysate.

性能

应用

Our Abpromise guarantee covers the use of ab2035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 76 kDa (predicted molecular weight: 80.5 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Essential adapter molecule for the activation of NF-kappa-B. Following different upstream signals (binding of inflammatory cytokines, stimulation of pathogen recognition receptors, or DNA damage), particular RIPK1-containing complexes are formed, initiating a limited number of cellular responses. Upon TNFA stimulation RIPK1 is recruited to a TRADD-TRAF complex initiated by TNFR1 trimerization. There, it is ubiquitinated via 'Lys-63'-link chains, inducing its association with the IKK complex, and its activation through NEMO binding of polyubiquitin chains.
  • 序列相似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 1 death domain.
    Contains 1 protein kinase domain.
  • 翻译后修饰Proteolytically cleaved by caspase-8 during TNF-induced apoptosis. Cleavage abolishes NF-kappa-B activation and enhances pro-apototic signaling through the TRADD-FADD interaction.
    Autophosphorylated on serine and threonine residues.
    Ubiquitinated by 'Lys-11'-, 'Lys-48'-, 'Lys-63'- and linear-linked type ubiquitin. Polyubiquitination with 'Lys-63'-linked chains by TRAF2 induces association with the IKK complex. Deubiquitination of 'Lys-63'-linked chains and polyubiquitination with 'Lys-48'-linked chains by TNFAIP3 leads to RIPK1 proteasomal degradation and consequently to the termination of the TNF- or Linear polyubiquitinated; the head-to-tail polyubiquitination is mediated by the LUBAC complex. LPS-mediated activation of NF-kappa-B. Also ubiquitinated with 'Lys-11'-linked chains.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cell death protein RIP antibody
    • FLJ39204 antibody
    • OTTHUMP00000039163 antibody
    • Receptor (TNFRSF) interacting serine threonine kinase 1 antibody
    • receptor interacting protein 1 antibody
    • Receptor interacting protein antibody
    • Receptor interacting protein kinase 1 antibody
    • Receptor interacting serine threonine protein kinase 1 antibody
    • Receptor TNFRSF interacting serine threonine kinase 1 antibody
    • Receptor-interacting protein 1 antibody
    • Receptor-interacting serine/threonine-protein kinase 1 antibody
    • Rinp antibody
    • RIP 1 antibody
    • RIP antibody
    • Rip-1 antibody
    • RIP1 antibody
    • RIPK 1 antibody
    • Ripk1 antibody
    • RIPK1_HUMAN antibody
    • Serine threonine protein kinase RIP antibody
    • Serine/threonine-protein kinase RIP antibody
    see all

Anti-RIP antibody 图像



  • Predicted band size : 80.5 kDa
    Western blot analysis of HepG2 cell lysate labeling RIP with ab2035 at 0.2-1 µg/ml.


  • Predicted band size : 80.5 kDa
    ab2035 at 1/1000 dilution staining approximately 76kDa band of human RIPK1 in human muscle lysate by Western blot (ECL). ab2035 at 1/1000 dilution staining approximately 76kDa band of human RIPK1 in human muscle lysate by Western blot (ECL).

Anti-RIP antibody (ab2035)参考文献

ab2035 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. I believe the image on the datasheet was labeled incorrectly so I have changed the datasheet. The antibody has only been tested in human. The immunogen only has 57% identity with the mouse sequence (8 of 14 amino acids ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"