概述

  • 产品名称Anti-RIP抗体
    参阅全部 RIP 一抗
  • 描述
    兔多克隆抗体to RIP
  • 特异性ab106393 reacts with RIP. It does not crossreact with other TKL Ser/Thr protein kinase family members.
  • 经测试应用适用于: IHC, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human RIP (N terminal).
    Database link: Q13546

  • 阳性对照
    • WB: Rat kidney lysate IHC-P: Mouse kidney tissue ICC/IF: Mouse kidney cells.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab106393 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC Use a concentration of 2.5 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 76 kDa.

靶标

  • 功能Essential adapter molecule for the activation of NF-kappa-B. Following different upstream signals (binding of inflammatory cytokines, stimulation of pathogen recognition receptors, or DNA damage), particular RIPK1-containing complexes are formed, initiating a limited number of cellular responses. Upon TNFA stimulation RIPK1 is recruited to a TRADD-TRAF complex initiated by TNFR1 trimerization. There, it is ubiquitinated via 'Lys-63'-link chains, inducing its association with the IKK complex, and its activation through NEMO binding of polyubiquitin chains.
  • 序列相似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 1 death domain.
    Contains 1 protein kinase domain.
  • 翻译后修饰Proteolytically cleaved by caspase-8 during TNF-induced apoptosis. Cleavage abolishes NF-kappa-B activation and enhances pro-apototic signaling through the TRADD-FADD interaction.
    Autophosphorylated on serine and threonine residues.
    Ubiquitinated by 'Lys-11'-, 'Lys-48'-, 'Lys-63'- and linear-linked type ubiquitin. Polyubiquitination with 'Lys-63'-linked chains by TRAF2 induces association with the IKK complex. Deubiquitination of 'Lys-63'-linked chains and polyubiquitination with 'Lys-48'-linked chains by TNFAIP3 leads to RIPK1 proteasomal degradation and consequently to the termination of the TNF- or Linear polyubiquitinated; the head-to-tail polyubiquitination is mediated by the LUBAC complex. LPS-mediated activation of NF-kappa-B. Also ubiquitinated with 'Lys-11'-linked chains.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cell death protein RIP antibody
    • FLJ39204 antibody
    • OTTHUMP00000039163 antibody
    • Receptor (TNFRSF) interacting serine threonine kinase 1 antibody
    • receptor interacting protein 1 antibody
    • Receptor interacting protein antibody
    • Receptor interacting protein kinase 1 antibody
    • Receptor interacting serine threonine protein kinase 1 antibody
    • Receptor TNFRSF interacting serine threonine kinase 1 antibody
    • Receptor-interacting protein 1 antibody
    • Receptor-interacting serine/threonine-protein kinase 1 antibody
    • Rinp antibody
    • RIP 1 antibody
    • RIP antibody
    • Rip-1 antibody
    • RIP1 antibody
    • RIPK 1 antibody
    • Ripk1 antibody
    • RIPK1_HUMAN antibody
    • Serine threonine protein kinase RIP antibody
    • Serine/threonine-protein kinase RIP antibody
    see all

Anti-RIP antibody 图像

  • Anti-RIP antibody (ab106393) at 1 µg/ml + Rat kidney tissue lysate at 15 µg

    Predicted band size : 76 kDa
  • Immunohistochemical analysis of mouse kidney tissue section labelling RIP with ab106393 at 2.5 ug/mL.

  • Immunofluorescence of RIP in Mouse Kidney cells using ab106393 at 20 ug/ml.

Anti-RIP antibody (ab106393)参考文献

This product has been referenced in:
  • Jackson R  et al. Paracrine Engineering of Human Cardiac Stem Cells With Insulin-Like Growth Factor 1 Enhances Myocardial Repair. J Am Heart Assoc 4:e002104 (2015). WB . Read more (PubMed: 26363004) »

See 1 Publication for this product

Product Wall

The immunogen is between amino acids 181-240 of accession number NP_003795.

Thank you for your call on Friday and for letting us know about the trouble with this antibody.

I've checked with the lab, and unfortunately we are not aware of any publications in which this antibody was used. Would you be able to forward me...

Read More
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (p7 mouse brain)
Specification p7 mouse brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

提交于 May 14 2012

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Kidney and brain)
Specification Kidney and brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acd
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

提交于 May 14 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"