Cow, Dog, Pig, Chimpanzee, Gorilla, Orangutan, Bat, Elephant
Synthetic peptide mapping to a region between residue 1 and 50 of human RIOK1 using the numbering given in entry (NP_113668.2)
Whole cell lysate from HeLa or 293T cells
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline, pH 7-8
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 66 kDa.
Use at 2-5 µg/mg of lysate.
Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.
Contains 1 protein kinase domain.
Information by UniProt
RIO kinase 1 (yeast) antibody
Western blot - Anti-RIOK1 antibody (ab88496)
All lanes : Anti-RIOK1 antibody (ab88496) at 0.04 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Predicted band size : 66 kDa Exposure time : 3 minutes
Immunoprecipitation - Anti-RIOK1 antibody (ab88496)
3ug ab88496 were used to immunoprecipitate 1mg HeLa whole cell lysate. 20% of immunoprecipitate was loaded on gel and probed with ab88496 at 0.4ug/ml.
Detection: chemoluminescence with exposure time of 30 seconds.
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