概述
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产品名称Anti-RING2 / RING1B / RNF2抗体
参阅全部 RING2 / RING1B / RNF2 一抗 -
描述兔多克隆抗体to RING2 / RING1B / RNF2
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经测试应用适用于: IPmore details
不适用于: WB -
种属反应性与反应: Human
预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
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免疫原
Synthetic peptide corresponding to a region between residue 286 and 336 of Human RING2/ RING1B/ RNF2 (NP_009143.1).
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阳性对照
- K562 whole cell lysate
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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存储溶液Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate buffer, pH 7-8 -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab101274 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | Use at 2-5 µg/mg of lysate. |
应用说明
Is unsuitable for WB.
靶标
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功能E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random X inactivation. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. E3 ubiquitin-protein ligase activity is enhanced by BMI1/PCGF4. Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity.
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通路Protein modification; protein ubiquitination.
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序列相似性Contains 1 RING-type zinc finger.
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翻译后修饰Polyubiquitinated in the presence of UBE2D3 (in vitro).
Monoubiquitinated, by auto-ubiquitination. -
细胞定位Nucleus. Chromosome. Enriched on inactive X chromosome (Xi) in female trophoblast stem (TS) cells as well as differentiating embryonic stem (ES) cells. The enrichment on Xi is transient during TS and ES cell differentiation. The association with Xi is mitotically stable in non-differentiated TS cells.
- Information by UniProt
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数据库链接
- Entrez Gene: 540090 Cow
- Entrez Gene: 480042 Dog
- Entrez Gene: 6045 Human
- Entrez Gene: 19821 Mouse
- Entrez Gene: 100174504 Orangutan
- Entrez Gene: 304850 Rat
- Omim: 608985 Human
- SwissProt: Q99496 Human
see all -
别名
- BAP 1 antibody
- BAP1 antibody
- DING antibody
see all
Anti-RING2 / RING1B / RNF2 antibody 图像
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Immunoprecipitation - RING2 / RING1B / RNF2 antibody (ab101274)Detection of RING2 / RING1B / RNF2 in Immunoprecipitates of K562 whole cell lysate (1 mg for IP, 20% of IP loaded) using ab101274 at 3 µg/mg lysate for IP. An anti-RING2 / RING1B / RNF2 antibody which recognizes an upstream epitope was used at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 30 seconds.
Anti-RING2 / RING1B / RNF2 antibody (ab101274)参考文献
ab101274 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"