概述

  • 产品名称Anti-RING1抗体
    参阅全部 RING1 一抗
  • 描述
    兔多克隆抗体to RING1
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 150-200 of Human RING1 (NP_002922.2).

  • 阳性对照
    • HeLa, 293T and Jurkat whole cell lysates.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7-8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab125193 was affinity purified using an epitope specific to RING1 immobilized on a solid support.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab125193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 42 kDa.
IP Use at 2-10 µg/mg of lysate.

靶标

  • 功能Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Contains 1 RING-type zinc finger.
  • 细胞定位Nucleus speckle.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Ring1A antibody
    • E3 ubiquitin-protein ligase RING1 antibody
    • Polycomb complex protein RING1 antibody
    • Really interesting new gene 1 protein antibody
    • RING finger protein 1 antibody
    • Ring1 antibody
    • RING1_HUMAN antibody
    • RING1A antibody
    • Rnf1 antibody
    • Transcription repressor Ring1A antibody
    see all

Anti-RING1 antibody 图像

  • All lanes : Anti-RING1 antibody (ab125193) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 42 kDa


    Exposure time : 3 minutes
  • Detection of RING1 by Western Blot of Immunprecipitate.
    ab125193 at 1µg/ml staining RING1 in HeLa whole cell lysate immunoprecipitated using ab125193 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 10 seconds.

Anti-RING1 antibody (ab125193)参考文献

ab125193 has not yet been referenced specifically in any publications.

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