Anti-RING finger protein unkempt like抗体(ab68731)

概述

  • 产品名称Anti-RING finger protein unkempt like抗体
    参阅全部 RING finger protein unkempt like 一抗
  • 描述
    小鼠多克隆抗体to RING finger protein unkempt like
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    RING finger protein unkempt like (NP_001032202, 1 a.a. ~ 277 a.a) full-length human protein.

  • 阳性对照
    • RING finger protein unkempt like transfected 293T cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab68731 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 31 kDa).

靶标

  • 功能May participate in a protein complex showing an E3 ligase activity regulated by RAC1. Ubiquitination is directed towards itself and possibly other substrates, such as SMARCD2/BAF60b. Intrinsic E3 ligase activity has not been proven.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Belongs to the unkempt family.
    Contains 4 C3H1-type zinc fingers.
    Contains 1 RING-type zinc finger.
  • 结构域Although this protein contains a RING domain, intrinsic E3 ligase activity has not been proven (PubMed:20148946).
  • 翻译后修饰Isoform 4 is ubiquitinated in the C-terminal. Ubiquitination is enhanced by activated RAC1. The presence of the RING finger domain is not essential for ubiquitination to occur.
  • 细胞定位Cytoplasm. Nucleus. Isoform 4 is primarily localized in the cytoplasm but has the ability to shuttle between the nucleus and the cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C16orf28 antibody
    • FLJ12623 antibody
    • FLJ23360 antibody
    • KIAA0734 antibody
    • MGC5179 antibody
    • Putative E3 ubiquitin-protein ligase UNKL antibody
    • RING finger protein unkempt-like antibody
    • Unkempt homolog (Drosophila) like antibody
    • Unkempt like antibody
    • Unkempt like protein antibody
    • Unkl antibody
    • UNKL_HUMAN antibody
    • ZC3H5L antibody
    • ZC3HDC5L antibody
    • Zinc finger CCCH domain-containing protein 5-like antibody
    see all

Anti-RING finger protein unkempt like antibody 图像

  • All lanes : Anti-RING finger protein unkempt like antibody (ab68731) at 1/500 dilution

    Lane 1 : RING finger protein unkempt like transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 31 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)

Anti-RING finger protein unkempt like antibody (ab68731)参考文献

ab68731 has not yet been referenced specifically in any publications.

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