概述

  • 产品名称Anti-RIN1抗体
    参阅全部 RIN1 一抗
  • 描述
    小鼠多克隆抗体to RIN1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    RIN1 human protein (NP_004283).

  • 阳性对照
    • RIN1 transfected 293T cell lysate, rat brain lysate

性能

应用

Our Abpromise guarantee covers the use of ab69897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: 1/500 - 1/1000. Detects bands of approximately 87 and 100 kDa in two separate blots (predicted molecular weight: 84 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性RIN1 is a Ras effector protein which can affect Ras signaling in a variety of ways. It enhances signaling from ABL1 and ABL2 whcih are involved in regulation of cytoskeletal remodeling. It may function as a guanine nucleotide exchange factor in activation of Rab5a. Rin1 also competes with RAF1 for binding of the activated form of Ras. It may play a role inhibiting the modulation of neuronal plasticity in formation of aversive memory, and has been implicated as a tumor supressor gene in breast cancer.
    • 细胞定位Cytoplasmic and Plasma membrane
    • 数据库链接
    • 别名
      • Ras and Rab interactor 1 antibody
      • ras inhibitor 1 antibody
      • ras inhibitor antibody
      • Ras inhibitor JC99 antibody
      • ras inhibitor RIN1 antibody
      • Ras interaction/interference protein 1 antibody
      • RIN 1 antibody
      see all

    Anti-RIN1 antibody 图像

    • Anti-RIN1 antibody (ab69897) at 1/500 dilution + rat brain at 25 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 84 kDa
      Observed band size : 87 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
    • All lanes : Anti-RIN1 antibody (ab69897) at 1/500 dilution

      Lane 1 : RIN1 transfected 293T cell lysate
      Lane 2 : Non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 84 kDa
      Observed band size : 100 kDa (why is the actual band size different from the predicted?)

    Anti-RIN1 antibody (ab69897)参考文献

    This product has been referenced in:
    • Kiel C  et al. Integration of protein abundance and structure data reveals competition in the ErbB signaling network. Sci Signal 6:ra109 (2013). Read more (PubMed: 24345680) »

    See 1 Publication for this product

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