• 产品名称Anti-Rif1抗体
    参阅全部 Rif1 一抗
  • 描述
    兔多克隆抗体to Rif1
  • 特异性Reactivity with mouse Rif1 is not expected owing to divergence of sequence between this ortholog and human Rif1 at the site to which the epitope maps.
  • 经测试应用适用于: IHC-P, IPmore details
    不适用于: WB
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 1 and 50 of human Rif1 (NP_060621.3)

  • 阳性对照
    • Whole cell lysate from 293T cells.



Our Abpromise guarantee covers the use of ab70254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 1-5 µg/mg of lysate.
  • 应用说明Is unsuitable for WB.
  • 靶标

    • 功能Required for checkpoint mediated arrest of cell cycle progression in response to DNA damage during S-phase (the intra-S-phase checkpoint). This checkpoint requires activation of at least 2 parallel pathways by the ATM kinase: one involves the MRN (MRE11A-RAD50-NBS1) complex, while the second requires CHEK2. RIF1 seems to act independently of both these pathways. Seems to play no role in either the G1/S or G2/M DNA damage checkpoints.
    • 组织特异性Highly expressed in testis.
    • 发展阶段Expression peaks in late G2/S phase of the cell cycle.
    • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位Nucleus. Chromosome > telomere. Cytoplasm > cytoskeleton > spindle. Exhibits ATM- and TP53BP1-dependent localization to uncapped or aberrant telomeres and to DNA double strand breaks (DSBs). Does not associate with normal telomere structures. Localizes to microtubules of the midzone of the mitotic spindle during anaphase, and to condensed chromosomes in telophase.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DKFZp781N1478 antibody
      • FLJ10599 antibody
      • FLJ11269 antibody
      • FLJ12870 antibody
      • Rap1 interacting factor 1 antibody
      • Rap1 interacting factor 1 homolog antibody
      • RAP1 interacting factor homolog antibody
      • RAP1 interacting factor homolog (yeast) antibody
      • Rap1-interacting factor 1 homolog antibody
      • Receptor interacting factor 1 antibody
      • RIF 1 antibody
      • RIF1 antibody
      • RIF1 isoform 4 antibody
      • RIF1_HUMAN antibody
      • Telomere associated protein RIF 1 antibody
      • Telomere associated protein RIF1 antibody
      • Telomere-associated protein RIF1 antibody
      • Telomeric protein RIF 1 antibody
      • Telomeric protein RIF1 antibody
      see all

    Anti-Rif1 antibody 图像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human laryngeal squamous cell carcinoma tissue labelling Rif1 with ab70254 at 1/1000 (1µg/ml). Detection: DAB.
    • Immunoprecipitation analysis of 293T whole cell lysate (1mg for IP, 25% of reaction loaded), using ab70254 at 5 µg/mg lysate for IP. Immunoprecipitated Rif1 was blotted using ab70307 at 0.4µg/ml.

    Anti-Rif1 antibody (ab70254)参考文献

    ab70254 has not yet been referenced specifically in any publications.

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    Please find below the details about the IP experiments for ab70254:

    IP lysis Buffer (also used as wash buffer)

    12.5 ml 1M NaCl (250mM)

    2.5 ml 1M Tris (50mM)


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