概述

  • 产品名称Anti-RICTOR抗体
    参阅全部 RICTOR 一抗
  • 描述
    兔多克隆抗体to RICTOR
  • 特异性We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 1 and 50 of human RICTOR.

  • 阳性对照
    • Whole cell lysate from HeLa cells.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab70373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 192 kDa (predicted molecular weight: 192 kDa).
IP Use at 3-5 µg/mg of lysate.

靶标

  • 功能Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development.
  • 序列相似性Belongs to the RICTOR family.
  • 翻译后修饰Phosphorylated by MTOR; when part of mTORC2. Phosphorylated at Thr-1135 by RPS6KB1; phosphorylation of RICTOR inhibits mTORC2 and AKT1 signaling.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AVO3 antibody
    • AVO3 homolog antibody
    • DKFZp686B11164 antibody
    • hAVO3 antibody
    • KIAA1999 antibody
    • Likely ortholog of mouse TORC2 specific protein AVO3 (S. cerevisiae) antibody
    • mAVO3 antibody
    • MGC39830 antibody
    • PIA antibody
    • Pianissimo antibody
    • Rapamycin insensitive companion of mTOR antibody
    • Rapamycin-insensitive companion of mTOR antibody
    • Rictor antibody
    • RICTR antibody
    • RICTR_HUMAN antibody
    • RPTOR independent companion of MTOR complex 2 antibody
    • TORC2 specific protein AVO3 antibody
    see all

Anti-RICTOR antibody 图像

  • All lanes : Anti-RICTOR antibody (ab70373) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg


    Predicted band size : 192 kDa
    Observed band size : 192 kDa
    Additional bands at : 160 kDa,300 kDa,80 kDa. We are unsure as to the identity of these extra bands.

Anti-RICTOR antibody (ab70373)参考文献

This product has been referenced in:
  • Yu Y & Alwine JC Interaction between simian virus 40 large T antigen and insulin receptor substrate 1 is disrupted by the K1 mutation, resulting in the loss of large T antigen-mediated phosphorylation of Akt. J Virol 82:4521-6 (2008). Read more (PubMed: 18305032) »

See 1 Publication for this product

Product Wall

rply. Please find here below the discount code for testing the Anti-SIN1 antibody (ab71152) on Zebra fish.

I have checked also for our other antibodies against Rictor.

We do have 8 antibodies tested in Western blot against Rictor, p...

Read More

Thank you for your inquiry and your interest in our antibodies!

I have contacted the laboratory to find out whether the immunogen sequence is similar to the Danio rerio sequence. The immunogen sequence is proprietary information, however the ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"