概述

  • 产品名称Anti-RICTOR抗体[7B3]
    参阅全部 RICTOR 一抗
  • 描述
    小鼠单克隆抗体[7B3] to RICTOR
  • 经测试应用适用于: WB, IHC-P, Flow Cyt, ICC/IF, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human, Monkey
  • 免疫原

    Purified recombinant fragment of Human RICTOR expressed in E. Coli.

  • 阳性对照
    • HeLa, PANC-1, MOLT4, HepG2 and HEK293 cell lysates. Thyroid gland tissues and Human breast carcinoma. NIH3T3 cells.

性能

应用

Our Abpromise guarantee covers the use of ab104838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2000. Predicted molecular weight: 192 kDa.
IHC-P 1/200 - 1/1000.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/200 - 1/1000.
ELISA 1/10000.

靶标

  • 功能Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development.
  • 序列相似性Belongs to the RICTOR family.
  • 翻译后修饰Phosphorylated by MTOR; when part of mTORC2. Phosphorylated at Thr-1135 by RPS6KB1; phosphorylation of RICTOR inhibits mTORC2 and AKT1 signaling.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AVO3 antibody
    • AVO3 homolog antibody
    • DKFZp686B11164 antibody
    • hAVO3 antibody
    • KIAA1999 antibody
    • Likely ortholog of mouse TORC2 specific protein AVO3 (S. cerevisiae) antibody
    • mAVO3 antibody
    • MGC39830 antibody
    • PIA antibody
    • Pianissimo antibody
    • Rapamycin insensitive companion of mTOR antibody
    • Rapamycin-insensitive companion of mTOR antibody
    • Rictor antibody
    • RICTR antibody
    • RICTR_HUMAN antibody
    • RPTOR independent companion of MTOR complex 2 antibody
    • TORC2 specific protein AVO3 antibody
    see all

Anti-RICTOR antibody [7B3] 图像

  • All lanes : Anti-RICTOR antibody [7B3] (ab104838) at 1/500 dilution

    Lane 1 : HeLa cell lysate.
    Lane 2 : PANC-1 cell lysate.
    Lane 3 : MOLT4 cell lysate.
    Lane 4 : HepG2 cell lysate.
    Lane 5 : HEK293 cell lysate.


    Predicted band size : 192 kDa
  • ab104838 at 1/200 dilution staining RICTOR in NIH3T3 cells (green) by immunofluorescence. DRAQ5 fluorescent DNA dye is shown in blue. Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red).
  • ab104838 at 1/200 dilution staining RICTOR in paraffin-embedded thyroid gland tissues by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab104838 at 1/200 dilution staining RICTOR in paraffin-fixed Human breast carcinoma by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab104838 used for Flow cytometric analysis of HeLa cells (green). The negative control is shown in purple.

Anti-RICTOR antibody [7B3] (ab104838)参考文献

This product has been referenced in:
  • Hütt-Cabezas M  et al. Activation of mTORC1/mTORC2 signaling in pediatric low-grade glioma and pilocytic astrocytoma reveals mTOR as a therapeutic target. Neuro Oncol 15:1604-14 (2013). IHC-P ; Human . Read more (PubMed: 24203892) »

See 1 Publication for this product

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