Anti-Ricin toxin A chain抗体(ab27169)

概述

  • 产品名称Anti-Ricin toxin A chain抗体
  • 描述
    兔多克隆抗体to Ricin toxin A chain
  • 特异性When tested by Western blotting on denatured RCA 60, this antibody reacts with only one band - the Ricin A chain.
  • 经测试应用适用于: Electron Microscopy, ELISA, WBmore details
  • 种属反应性
    Reacts with Ricinus communis. Not yet tested in other species.
  • 免疫原

    Full length native protein: Purified intact A chain from RCA 60.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 15mM Sodium Azide.
    Constituents: 0.5M Sodium chloride, 0.01M PBS, pH 7.4.
  • 纯度Whole antiserum
  • 纯化说明sterile-filtered (0.22 mm pore size) serum with the addition of 15 mM NaN3
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab27169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Electron Microscopy Use at an assay dependent concentration. PubMed: 21886775
ELISA 1/20000.
WB 1/2000. Predicted molecular weight: 32 kDa.

靶标

  • 相关性Ricin chain A (RTA) is a ribosome inactivating enzyme with a molecular weight of 32 kDa. RTA is normally linked by a disulfide bond to the galactose/N acetylgalactosamine binding lectin (34 kDa), also called the B chain or RTB. Together with RTB, RTA constitutes the heterodimeric cytotoxin Ricin (RCA 60) from Ricinus communis. This ricin, which is a type 2 RIP (ribosome inactivating protein) is among the most potent cytotoxins in nature. Ricin is highly toxic to animal cells and to a lesser extent to plant cells.
  • 别名
    • Ricin A chain antibody
    • Ricin antibody

Anti-Ricin toxin A chain antibody (ab27169)参考文献

This product has been referenced in:
  • Wang W  et al. Mannosidase 2, alpha 1 Deficiency Is Associated with Ricin Resistance in Embryonic Stem (ES) Cells. PLoS One 6:e22993 (2011). Electron Microscopy . Read more (PubMed: 21886775) »
  • Ray S  et al. Modulation of toxin stability by 4-phenylbutyric Acid and negatively charged phospholipids. PLoS One 6:e23692 (2011). Read more (PubMed: 21887297) »

See all 3 Publications for this product

Product Wall

I have found an article on the Internet which indicates very different structures between Ricin Communis Agglutinin and Ricin (toxin). Biol Chem. 1985 Dec 15;260(29):15682-6. The primary sequence of Ricinus communis agglutinin. Comparison with ri...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"