Anti-RIC8A抗体(ab97808)
Key features and details
- Rabbit polyclonal to RIC8A
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RIC8A抗体
参阅全部 RIC8A 一抗 -
描述
兔多克隆抗体to RIC8A -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to a region within amino acids 528 and 537 of Human RIC8A (NP_068751).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97808于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/100 - 1/500.
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WB |
1/500 - 1/3000. Predicted molecular weight: 60 kDa.
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说明 |
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IHC-P
1/100 - 1/500. |
WB
1/500 - 1/3000. Predicted molecular weight: 60 kDa. |
靶标
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功能
Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex (By similarity). Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation. -
序列相似性
Belongs to the synembryn family. -
细胞定位
Cytoplasm. Cell membrane. Colocalizes with RIC8A in CA2 hippocampal neurons. Colocalizes with GNAI1 and RGS14 at the plasma membrane (By similarity). - Information by UniProt
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数据库链接
- Entrez Gene: 60626 Human
- Omim: 609146 Human
- SwissProt: Q9NPQ8 Human
- Unigene: 592292 Human
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别名
- MGC104517 antibody
- MGC131931 antibody
- MGC148073 antibody
see all
图片
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All lanes : Anti-RIC8A antibody (ab97808) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 60 kDa
7.5% SDS PAGE -
ab97808, at a 1/100 dilution, staining RIC8A in paraffin embedded Adrenal cortical tumor by Immunohistochemistry.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab97808 被引用在 3 文献中.
- Zeng H et al. Genome-wide CRISPR screening reveals genetic modifiers of mutant EGFR dependence in human NSCLC. Elife 8:N/A (2019). PubMed: 31741433
- Chishiki K et al. Ric-8A, an activator protein of Gai, controls mammalian epithelial cell polarity for tight junction assembly and cystogenesis. Genes Cells 22:293-309 (2017). PubMed: 28185378
- Boularan C et al. Resistance to inhibitors of cholinesterase (Ric)-8A and Gai contribute to cytokinesis abscission by controlling vacuolar protein-sorting (Vps)34 activity. PLoS One 9:e86680 (2014). WB, IP, ICC/IF ; Human . PubMed: 24466196