概述

  • 产品名称
  • 描述
    兔多克隆抗体to RIC8A
  • 经测试应用
    适用于: ICC/IF, IP, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to a region within amino acids 528 and 537 of Human RIC8A (NP_068751).

  • 阳性对照
    • A431, H1299, HeLa, HepG2 and Raji cell lines; Adrenal cortical tumor

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab97808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 24466196
IP Use at an assay dependent concentration. PubMed: 24466196
IHC-P 1/100 - 1/500.
WB 1/500 - 1/3000. Predicted molecular weight: 60 kDa.

靶标

  • 功能
    Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex (By similarity). Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation.
  • 序列相似性
    Belongs to the synembryn family.
  • 细胞定位
    Cytoplasm. Cell membrane. Colocalizes with RIC8A in CA2 hippocampal neurons. Colocalizes with GNAI1 and RGS14 at the plasma membrane (By similarity).
  • Information by UniProt
  • 数据库链接
  • 别名
    • MGC104517 antibody
    • MGC131931 antibody
    • MGC148073 antibody
    • MGC148074 antibody
    • Protein Ric-8A antibody
    • resistance to inhibitors of cholinesterase 8 homolog A (C. elegans) antibody
    • RIC8 antibody
    • RIC8A antibody
    • RIC8A_HUMAN antibody
    • Synembryn-A antibody
    see all

图片

  • All lanes : Anti-RIC8A antibody (ab97808) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : HeLa whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 60 kDa
  • ab97808, at a 1/100 dilution, staining RIC8A in paraffin embedded Adrenal cortical tumor by Immunohistochemistry.

文献

This product has been referenced in:
  • Boularan C  et al. Resistance to inhibitors of cholinesterase (Ric)-8A and Gai contribute to cytokinesis abscission by controlling vacuolar protein-sorting (Vps)34 activity. PLoS One 9:e86680 (2014). WB, IP, ICC/IF ; Human . Read more (PubMed: 24466196) »

See 1 Publication for this product

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