Anti-ribosomal RNA 5.8s抗体[Y10b] (ab37144)

概述

  • 产品名称Anti-ribosomal RNA 5.8s抗体[Y10b]
    参阅全部 ribosomal RNA 5.8s 一抗
  • 描述
    小鼠单克隆抗体[Y10b] to ribosomal RNA 5.8s
  • 特异性This antibody is specific for ribosomal RNA. There is no cross-reactivity with other RNAs.
  • 经测试应用适用于: IP, ICC/IFmore details
  • 种属反应性
    This antibody reacts with all eukaryotes.
  • 免疫原

    The whole 5.8S ribosomal RNA.

  • 表位This antibody is useful for recognizing RNA epitopes on both the large and small subunit.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.05% Sodium Azide
  • Concentration information loading...
  • 纯度Protein A purified
  • 纯化说明Protein A purified mouse ascites.
  • 克隆单克隆
  • 克隆编号Y10b
  • 同种型IgG3
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab37144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.

靶标

  • 相关性The inhibition of protein synthesis by specific anti 5.8S rRNA oligonucleotides have suggested that this RNA plays an important role in eukaryotic ribosome function, specifically ribosomal translocation. Mutations in the 5.8S rRNA can inhibit cell growth and compromise protein synthesis in vitro.
  • 细胞定位Cytoplasmic and Nuclear

Anti-ribosomal RNA 5.8s antibody [Y10b] (ab37144)参考文献

This product has been referenced in:
  • Zhu CH  et al. SGNP: an essential Stress Granule/Nucleolar Protein potentially involved in 5.8s rRNA processing/transport. PLoS ONE 3:e3716 (2008). ICC/IF ; Human . Read more (PubMed: 19005571) »
  • Thomas MG  et al. Staufen recruitment into stress granules does not affect early mRNA transport in oligodendrocytes. Mol Biol Cell 16:405-20 (2005). Read more (PubMed: 15525674) »

See all 14 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Human Cell (Lymphoblast)
Specification Lymphoblast
Permeabilization Yes - 0.1% TX-100 for 10 min.
Fixative Paraformaldehyde
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提交于 Mar 26 2014

Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (brain)
Specification brain
Fixative paraformaldehyde + glutaraldehyde
Permeabilization No
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 22°C
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提交于 Dec 09 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"