Anti-Ribosomal protein S10抗体(ab95944)

概述

  • 产品名称
    Anti-Ribosomal protein S10抗体
    参阅全部 Ribosomal protein S10 一抗
  • 描述
    兔多克隆抗体to Ribosomal protein S10
  • 经测试应用
    适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Zebrafish
  • 免疫原

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 - 161 of Human Ribosomal protein S10 (NP_001005)

  • 阳性对照
    • 293T, A431, H1299, HepG2, MOLT4 and Raji cell lysates IF: HeLa cells IHC-P: A549 xenograft

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab95944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 19 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

靶标

  • 功能
    Component of the 40S ribosomal subunit.
  • 疾病相关
    Defects in RPS10 are the cause of Diamond-Blackfan anemia type 9 (DBA9) [MIM:613308]. It is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre- Robin syndrome and cleft palate), thumb and urogenital anomalies.
  • 序列相似性
    Belongs to the ribosomal protein S10e family.
  • 翻译后修饰
    Methylated by PRMT5. Methylation is necessary for its interaction with NPS1, its localization in the granular component (GC) region of the nucleolus, for the proper assembly of ribosomes, protein synthesis and optimal cell proliferation.
  • 细胞定位
    Cytoplasm. Nucleus > nucleolus. Localized in the granular component (GC) region of the nucleolus. Methylation is required for its localization in the GC region. Co-localizes with NPS1 in the GC region of the nucleolus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 40S ribosomal protein S10 antibody
    • DBA9 antibody
    • MGC88819 antibody
    • OTTHUMP00000016229 antibody
    • OTTHUMP00000016230 antibody
    • RPS10 antibody
    • RS10_HUMAN antibody
    • S10 antibody
    see all

图片

  • Anti-Ribosomal protein S10 antibody (ab95944) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size : 19 kDa
  • ab95944, at a 1/200 dilution, staining Ribosomal protein S10 in paraformaldehyde-fixed HeLa cells by Immunofluorescence analysis. Right image is merged with a DNA probe.
  • ab95944, at a 1/500 dilution, staining of Ribosomal protein S10 in a paraffin-embedded A549 xenograft by Immunohistochemical analysis.

文献

ab95944 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
PBS-1% ovalbumin as blocking agent for 20 minute(s) · Concentration: 1.0% · Temperature: 22°C
Sample
Salmo salar Cell (testicular)
Specification
testicular
Permeabilization
Yes - methyl methacrylate/Butyl methacrylate
Fixative
glutaraldehyde-formaldehyde
Username

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提交于 Mar 25 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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