Anti-Ribophorin I抗体(ab52672)

概述

  • 产品名称Anti-Ribophorin I抗体
    参阅全部 Ribophorin I 一抗
  • 描述
    小鼠多克隆抗体to Ribophorin I
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Saccharomyces cerevisiae
  • 免疫原

    Vector coding for a partial recombinant fusion protein, corresponding to internal sequence amino acids 629-728 of Saccharomyces cerevisiae Ribophorin I. [Swiss Prot P38764] Target sequence used to make the antibody: VKGEEDADEE ETAEGQTNSI SDFLGEQVNE PTKNEEAEIE VDEMEVDAEG EEVEVKAEIT EKKNGESLEG EEIKSEEKKG KSSDKDATTD GKNDDEEEEK.

  • 常规说明


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 50% Glycerol, Whole serum
  • 纯度Whole antiserum
  • Primary antibody说明This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab52672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 109 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

靶标

  • 功能Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.
  • 组织特异性Expressed in all tissues tested.
  • 序列相似性Belongs to the OST1 family.
  • 细胞定位Endoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 别名
    • D6Wsu137e antibody
    • Dolichyl diphosphooligosaccharide protein glycosyltransferase 67 kDa subunit antibody
    • Dolichyl diphosphooligosaccharide protein glycosyltransferase 67 kDa subunit precursor antibody
    • Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit antibody
    • Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 antibody
    • EC 2.4.1.119 antibody
    • Oligosaccharyltransferase 1 homolog antibody
    • oligosaccharyltransferase complex subunit (non-catalytic) antibody
    • OST1 antibody
    • RBPH1 antibody
    • Ribophorin I antibody
    • Ribophorin-1 antibody
    • RPN-I antibody
    • Rpn1 antibody
    • RPN1_HUMAN antibody
    see all

Anti-Ribophorin I antibody (ab52672)参考文献

ab52672 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Pichia pastoris Cell lysate - other (membrane fraction (sucrose gradient))
Loading amount 2 µg
Specification membrane fraction (sucrose gradient)
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Laura Baciou

Verified customer

提交于 Dec 01 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"