概述

  • 产品名称Anti-Ribonuclease T2抗体
    参阅全部 Ribonuclease T2 一抗
  • 描述
    兔多克隆抗体to Ribonuclease T2
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    KLH conjugated synthetic peptide between amino acids 13-40 from the N terminal region of Human Ribonuclease T2 (NP_003721.2).

  • 阳性对照
    • NCI-H460 cell line lysates; Human spleen carcinoma This antibody gave a positive result when used in the following formaldehyde fixed cell lines: LoVo

性能

应用

Our Abpromise guarantee covers the use of ab107313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB 1/100 - 1/1000. Predicted molecular weight: 29 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 组织特异性Ubiquitous. Higher expression levels observed in the temporal lobe and fetal brain.
  • 疾病相关Defects in RNASET2 are the cause of leukoencephalopathy cystic without megalencephaly (LCWM) [MIM:612951]. An infantile-onset syndrome of cerebral leukoencephalopathy. Affected newborns develop microcephaly and neurologic abnormalities including psychomotor impairment, seizures and sensorineural hearing impairment. The brain shows multifocal white matter lesions, anterior temporal lobe subcortical cysts, pericystic abnormal myelination, ventriculomegaly and intracranial calcifications.
  • 序列相似性Belongs to the RNase T2 family.
  • 细胞定位Secreted. Subcellular fractionation of transfected ovarian cancer cells reveals full-length RNASET2 in the endoplasmic reticulum fraction and the 2 smaller RNASET2 proteolytic products in the lysosome fraction.
  • Information by UniProt
  • 数据库链接
  • 别名
    • bA514O12.3 antibody
    • Ribonuclease 6 antibody
    • Ribonuclease T2 antibody
    • RNASE6PL antibody
    • Rnaset2 antibody
    • RNT2_HUMAN antibody
    • RP11 514O12.3 antibody
    see all

Anti-Ribonuclease T2 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen carcinoma tissue sections labeling Ribonuclease T2 with ab107313 at 1/25 dilution. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 37°C. Heat mediated antigen retrieval was performed using a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hour at 37°C. A Peroxidase-conjugated Goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

  • ab107313 stained LoVo cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab107313 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-Ribonuclease T2 antibody (ab107313) at 1/1000 dilution + NCI-H460 whole cell lysate at 35 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size : 29 kDa

    Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-Ribonuclease T2 antibody (ab107313)参考文献

This product has been referenced in:
  • Wang Q  et al. Stress-induced RNASET2 overexpression mediates melanocyte apoptosis via the TRAF2 pathway in vitro. Cell Death Dis 5:e1022 (2014). WB ; Human . Read more (PubMed: 24457966) »

See 1 Publication for this product

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