Anti-Ribonuclease A抗体(ab94417)

概述

  • 产品名称Anti-Ribonuclease A抗体
    参阅全部 Ribonuclease A 一抗
  • 描述
    兔多克隆抗体to Ribonuclease A
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Rabbit, Goat, Horse, Guinea pig, Cow, Cat, Pig, Chimpanzee, Drosophila melanogaster, African Green Monkey, Spider Monkey
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 108-157 (HITDCRLTNG SRYPNCAYRT SPKERHIIVA CEGSPYVPVH FDASVEDST) of Human Ribonuclease A (NP_002924).

  • 阳性对照
    • Human fetal kidney lysate IHC-P: Human lung FFPE tissue sections.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab94417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 18 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-Ribonuclease A antibody 图像

  • IHC image of Ribonuclease A staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab94417, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-Ribonuclease A antibody (ab94417) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal kidney lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 18 kDa
    Gel concentration: 10-20%

Anti-Ribonuclease A antibody (ab94417)参考文献

ab94417 has not yet been referenced specifically in any publications.

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