Anti-Ribonuclease 3抗体(ab116017)

概述

  • 产品名称
    Anti-Ribonuclease 3抗体
    参阅全部 Ribonuclease 3 一抗
  • 描述
    兔多克隆抗体to Ribonuclease 3
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 123-151 of Human Ribonuclease 3 (NP_002926.2).

  • 阳性对照
    • NCI-H460 cell line lysates This antibody gave a positive result in IHC in the following FFPE tissue: Human normal kidney.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • 存储溶液
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab116017 is purified through a protein A column, followed by peptide affinity purification.
  • 克隆
    多克隆
  • 同种型
    IgG

应用

Our Abpromise guarantee covers the use of ab116017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 18 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity.
  • 序列相似性
    Belongs to the pancreatic ribonuclease family.
  • 细胞定位
    Cytoplasmic granule. Matrix of eosinophil's large specific granule.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cytotoxic ribonuclease antibody
    • ECP antibody
    • ECP_HUMAN antibody
    • Eosinophil cationic protein antibody
    • OTTHUMP00000164017 antibody
    • Ribonuclease 3 antibody
    • Ribonuclease, RNase A family, 3 antibody
    • RNase 3 antibody
    • RNASE3 antibody
    • RNS3 antibody
    see all

图片

  • IHC image of Ribonuclease 3 staining in Human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab116017, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-Ribonuclease 3 antibody (ab116017) at 1/100 dilution + NCI-H460 cell line lysates at 35 µg

    Predicted band size : 18 kDa

文献

ab116017 has not yet been referenced specifically in any publications.

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