概述

  • 产品名称Anti-RIAM抗体[EPR2806]
    参阅全部 RIAM 一抗
  • 描述
    兔单克隆抗体[EPR2806] to RIAM
  • 经测试应用适用于: WB, IP, ICC, IHC-Pmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human RIAM aa 1-100 (N terminal).

  • 阳性对照
    • Human Lymph node, Fetal brain, Human spleen, Jurkat and HL-60 + retinoic acid cell lysates; Human tonsil tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab92537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
ICC
IHC-P
  • 应用说明ICC: 1/100 - 1/250.
    IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    IP: 1/50.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 73 kDa.

    Is unsuitable for Flow Cyt.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性RIAM (Rap1-GTP interacting adapter molecule) appears to function in the signal transduction from Ras activation to actin cytoskeletal remodeling via interaction with profilin and VASP. It suppresses insulin-induced promoter activities through AP1 and SRE and also mediates Rap1-induced adhesion.
    • 细胞定位Cell Membrane, Cytoplasm and cytoskeleton
    • 数据库链接
    • 别名
      • AB1IP antibody
      • Amyloid beta A4 precursor protein binding family B member 1 interacting protein antibody
      • Amyloid beta precursor protein binding family B member 1 antibody
      • APBB1 interacting protein 1 antibody
      • INAG1 antibody
      • OTTHUMP00000019344 antibody
      • PREL 1 antibody
      • PREL1 antibody
      • Proline rich EVH1 ligand 1 antibody
      • Proline rich protein 73 antibody
      • Rap1 GTP interacting adapter molecule antibody
      • RAP1 interacting adaptor molecule antibody
      • RARP 1 antibody
      • RARP1 antibody
      • Retinoic acid responsive proline rich protein 1 antibody
      see all

    Anti-RIAM antibody [EPR2806] 图像

    • All lanes : Anti-RIAM antibody [EPR2806] (ab92537) at 1/2000 dilution

      Lane 1 : Human Lymph node lysate
      Lane 2 : Fetal brain lysate
      Lane 3 : Human spleen lysate
      Lane 4 : Jurkat cell lysate
      Lane 5 : HL-60 + retinoic acid cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 73 kDa
    • ab92537, at 1/100 dilution, staining RIAM in paraffin-embedded Human tonsil tissue by Immunohistochemistry.

    Anti-RIAM antibody [EPR2806] (ab92537)参考文献

    This product has been referenced in:
    • Su W  et al. Rap1 and its effector RIAM are required for lymphocyte trafficking. Blood 126:2695-703 (2015). Read more (PubMed: 26324702) »
    • Wynne JP  et al. Rap1-interacting adapter molecule (RIAM) associates with the plasma membrane via a proximity detector. J Cell Biol 199:317-30 (2012). WB . Read more (PubMed: 23045549) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"