概述

  • 产品名称Anti-Rhp51抗体
  • 描述
    兔多克隆抗体to Rhp51
  • 经测试应用适用于: WB, IP, ICC/IFmore details
  • 种属反应性
    与反应: Schizosaccharomyces pombe
  • 免疫原

    Purified recombinant full length protein (Schizosaccharomyces pombe)

  • 阳性对照
    • Schizosaccharomyces pombe whole cell extracts.

性能

应用

Our Abpromise guarantee covers the use of ab63799 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/5000. Detects a band of approximately 44 kDa (predicted molecular weight: 40 kDa).
IP Use at an assay dependent concentration.
ICC/IF 1/500.

靶标

  • 相关性Rhp51 is required both for recombination and for the repair of DNA damage caused by X-rays. Binds to single and double stranded DNA, in the presence of magnesium, and exhibits DNA-dependent ATPase activity. Promotes DNA strand annealing and strand exchange via DNA recombinase activity and forms helical nucleoprotein filaments.
  • 细胞定位Nuclear
  • 数据库链接
    • 别名
      • DNA repair protein rhp51 antibody
      • Rad51 antibody
      • RAD51 homolog antibody
      • Recombinase Rhp51 antibody

    Anti-Rhp51 antibody 图像

    • ab63799 (1/500) staining Rhp51 in S. pombe cells without or with MMS (0.025%) treatment for 1 hour by ICC/IF (Immunocytochemistry/immunofluorescence).

    • All lanes : Anti-Rhp51 antibody (ab63799) at 1/2000 dilution

      Lane 1 : Molecular weight markers
      Lane 2 : Whole cell extract of Schizosaccharomyces pombe wild-type strain
      Lane 3 : Whole cell extract of Schizosaccharomyces pombe Rad51 deletion strain


      Predicted band size : 40 kDa
      Observed band size : 44 kDa (why is the actual band size different from the predicted?)

    Anti-Rhp51 antibody (ab63799)参考文献

    ab63799 has not yet been referenced specifically in any publications.

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