概述

  • 产品名称Anti-RhoGDI (phospho S174)抗体
    参阅全部 RhoGDI 一抗
  • 描述
    兔多克隆抗体to RhoGDI (phospho S174)
  • 特异性The antibody detects endogenous levels of RhoGDI only when phosphorylated at serine 174.
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthesized phosphopeptide derived from human RhoGDI around the phosphorylation site of serine 174 (R-G-SP-Y-S).

  • 阳性对照
    • IHC-P: Human cervix carcinoma tissue. WB: HUVEC cells treated with EGF.

性能

应用

Our Abpromise guarantee covers the use of ab74142 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 23 kDa.
ELISA 1/20000.
IHC-P 1/50 - 1/100.

靶标

  • 功能Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them.
  • 序列相似性Belongs to the Rho GDI family.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ARHGDIA antibody
    • fa96g11 antibody
    • GDIA 1 antibody
    • GDIA1 antibody
    • GDIR1_HUMAN antibody
    • MGC117248 antibody
    • NPHS8 antibody
    • Rho GDI 1 antibody
    • Rho GDI alpha antibody
    • Rho GDI antibody
    • Rho GDP dissociation inhibitor (GDI) alpha antibody
    • Rho GDP dissociation inhibitor 1 antibody
    • Rho GDP dissociation inhibitor alpha antibody
    • Rho GDP-dissociation inhibitor 1 antibody
    • Rho-GDI alpha antibody
    • RhoGDI 1 antibody
    • RhoGDI alpha antibody
    • RHOGDI antibody
    • RhoGDI1 antibody
    • wu:fa96g11 antibody
    • zgc:55554 antibody
    • zgc:77681 antibody
    see all

Anti-RhoGDI (phospho S174) antibody 图像

  • All lanes : Anti-RhoGDI (phospho S174) antibody (ab74142) at 1/500 dilution

    Lane 1 : HUVEC cells treated with EGF (200ng/ml, 30mins)
    Lane 2 : HUVEC cells treated with EGF (200ng/ml, 30mins) with phosphopeptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 23 kDa
    Observed band size : 23 kDa
    Additional bands at : 105 kDa,60 kDa. We are unsure as to the identity of these extra bands.
  • Human cervix carcinoma tissue stained with ab74142 (1/50 dilution).
    1: No phosphopeptide
    2: Phosphopeptide

Anti-RhoGDI (phospho S174) antibody (ab74142)参考文献

This product has been referenced in:
  • Oishi A  et al. Regulation of RhoA Signaling by the cAMP-dependent Phosphorylation of RhoGDIa. J Biol Chem 287:38705-15 (2012). WB . Read more (PubMed: 23012358) »

See 1 Publication for this product

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