概述

  • 产品名称Anti-RhoGAP抗体
    参阅全部 RhoGAP 一抗
  • 描述
    兔多克隆抗体to RhoGAP
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    KLH conjugated synthetic peptide selected from the center region of Human RhoGAP (NP_001164.2).

  • 阳性对照
    • 293 cell line lysate; Mouse cerebellum tissue lysates

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab93375 is purified through a protein A column, followed by peptide affinity purification.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab93375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明ELISA: 1/1000.
    WB: 1/100 - 1/500. Predicted molecular weight: 171 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性The p190 RhoGAP protein is associated with p120 RasGAP in growth-factor stimulated and tyrosine kinase transformed cells. It functions as a GTPase - activating protein (GAP) for Rho and Rac family proteins, which are involved in regulating cytoskeletal actin and membrane ruffling. The antibody appears to couple signal transduction via Ras and Rho through its association with RasGAP. The presence of two adjacent SH2 domains in the p21 RAS GAP indicates that GAP might interact directly with tyrosine kinases. p190B can be directly phosphorylated on a single identified tyrosine residue by the activated insulin and IGF-1 receptors. This phosphorylation causes p190B to translocate to the lipid rafts of the plasma membrane, where it can facilitate the inactivation of the Rho GTPase. P190B also has been shown to be tyrosine phosphorylated by cSrc and vSrc. Additionally the GAP domain of p190B is shown to attenuate signal transducing activity of Rac, Rho and CDC42.
    • 细胞定位Cytoplasmic
    • 数据库链接
    • 别名
      • ARHGAP 5 antibody
      • ARHGAP5 antibody
      • p190 B antibody
      • p190 RhoGAP antibody
      • Rho GTPase activating protein 5 antibody
      • RhoGAP 5 antibody
      • RhoGAP antibody
      • RhoGAP5 antibody
      see all

    Anti-RhoGAP antibody 图像

    • All lanes : Anti-RhoGAP antibody (ab93375) at 1/100 dilution

      Lane 1 : 293 cell line lysate
      Lane 2 : Mouse cerebellum tissue lysate

      Lysates/proteins at 35 µg per lane.


      Predicted band size : 171 kDa

    Anti-RhoGAP antibody (ab93375)参考文献

    ab93375 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"