概述

  • 产品名称Anti-RHOG抗体[2E6]
    参阅全部 RHOG 一抗
  • 描述
    小鼠单克隆抗体[2E6] to RHOG
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Hamster, Cow, Pig, Duck
  • 免疫原

    Recombinant fragment with tag (MW of the tag alone is 26 KDa): RHKWHPEVCH HCPDVPILLV GTKKDLRAQP DTLRRLKEQG QAPITPQQGQ ALAKQIHAV RYLECSALQQ DGVKEVFAEA VRAVLNPTPI KRGRSCILL, corresponding to amino acids 94-192 of Human RHOG. Note: the sequence provided does not include that of the tag.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 克隆编号2E6
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab76508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明ELISA: Use at an assay dependent dilution.
    sELISA: Use at an assay dependent dilution.
    WB: 1/500 - 1/1000. Predicted molecular weight: 21 kDa.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Required for the formation of membrane ruffles during macropinocytosis. Required for the formation of cup-like structures during trans-endothelial migration of leukocytes. In case of Salmonella enterica infection, activated by SopB and ARHGEF26/SGEF, which induces cytoskeleton rearrangements and promotes bacterial entry.
    • 序列相似性Belongs to the small GTPase superfamily. Rho family.
    • 细胞定位Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ARHG antibody
      • Ras homolog family member G antibody
      • Rho G antibody
      • Rho related GTP binding protein RhoG precursor antibody
      • Rho-related GTP-binding protein RhoG antibody
      • RHOG antibody
      • RHOG_HUMAN antibody
      see all

    Anti-RHOG antibody [2E6] 图像

    • Anti-RHOG antibody [2E6] (ab76508) at 1/500 dilution + immunogen at 0.2 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

      Predicted band size : 21 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    • Detection limit for recombinant tagged RHOG is approximately 1ng/ml when used as a capture antibody at 1ug/well.

    Anti-RHOG antibody [2E6] (ab76508)参考文献

    ab76508 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab76508.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"