Anti-RhoF抗体(ab101349)

概述

  • 产品名称Anti-RhoF抗体
    参阅全部 RhoF 一抗
  • 描述
    兔多克隆抗体to RhoF
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Cow, Pig
  • 免疫原

    Recombinant fragment, corresponding to a region within internal sequence amino acids 16-156 of Human RhoF (AAH18208).

  • 阳性对照
    • U87-MG whole cell lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human normal colon.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab101349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/3000. Predicted molecular weight: 24 kDa.

靶标

  • 功能Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Causes the formation of thin, actin-rich surface projections called filopodia. Functions cooperatively with CDC42 and Rac to generate additional structures, increasing the diversity of actin-based morphology.
  • 序列相似性Belongs to the small GTPase superfamily. Rho family.
  • 细胞定位Cell membrane. Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ARHF antibody
    • FLJ20247 antibody
    • Ras homolog gene family member F (in filopodia) antibody
    • Ras homolog gene family member F antibody
    • Rho F antibody
    • Rho family GTPase Rif antibody
    • Rho in filopodia antibody
    • Rho related GTP binding protein RhoF antibody
    • Rho-related GTP-binding protein RhoF antibody
    • Rhof antibody
    • RHOF_HUMAN antibody
    • RIF antibody
    see all

Anti-RhoF antibody 图像

  • IHC image of RhoF staining in Human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab101349, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-RhoF antibody (ab101349) at 1/500 dilution + U87-MG whole cell lysate at 30 µg

    Predicted band size : 24 kDa
    12% SDS PAGE

Anti-RhoF antibody (ab101349)参考文献

ab101349 has not yet been referenced specifically in any publications.

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