Anti-Rhodopsin抗体[Rho 4D2] (ab98887)


  • 产品名称Anti-Rhodopsin抗体[Rho 4D2]
    参阅全部 Rhodopsin 一抗
  • 描述
    小鼠单克隆抗体[Rho 4D2] to Rhodopsin
  • 经测试应用适用于: Flow Cyt, WB, IP, ELISA, IHC-P, IHC-Fr, ICCmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human, Pig, Xenopus laevis
    不与反应: Drosophila melanogaster
  • 免疫原

    Bovine Rhodopsin



Our Abpromise guarantee covers the use of ab98887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB 1/1000. Predicted molecular weight: 39 kDa.
IP Use at an assay dependent concentration. 1-2 µg per 100-500µg of protein.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.


  • 功能Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.
  • 组织特异性Rod shaped photoreceptor cells which mediates vision in dim light.
  • 疾病相关Retinitis pigmentosa 4
    Night blindness, congenital stationary, autosomal dominant 1
  • 序列相似性Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.
  • 翻译后修饰Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
    Contains one covalently linked retinal chromophore.
  • 细胞定位Membrane. Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CSNBAD1 antibody
    • MGC138309 antibody
    • MGC138311 antibody
    • OPN 2 antibody
    • OPN2 antibody
    • opsd antibody
    • OPSD_HUMAN antibody
    • opsin 2 antibody
    • Opsin 2 rod pigment antibody
    • Opsin-2 antibody
    • Opsin2 antibody
    • Retinitis Pigmentosa 4 antibody
    • Retinitis pigmentosa 4 autosomal dominant antibody
    • RHO antibody
    • Rhodopsin antibody
    • RP 4 antibody
    • RP4 antibody
    see all

Anti-Rhodopsin antibody [Rho 4D2] 图像

  • Overlay histogram showing Y79 cells stained with ab98887 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab98887, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Y79 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Rhodopsin antibody [Rho 4D2] (ab98887)参考文献

This product has been referenced in:
  • Sawant O  et al. Light-Regulated Thyroid Hormone Signaling Is Required for Rod Photoreceptor Development in the Mouse Retina. Invest Ophthalmol Vis Sci 56:8248-57 (2015). IF ; Mouse . Read more (PubMed: 26720479) »

See 1 Publication for this product

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Thank you for contacting Abcam and thank you for sending the details of your protocol as well as the image. These have been very helpful.

After reviewing your protocol, I would like to suggest a few tips which may help reduce the upper dark ar...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Retina)
Gel Running Conditions Reduced Denaturing (12% Gel)
Loading amount 2 µg
Specification Retina
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Onkar Sawant

Verified customer

提交于 Mar 04 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Eye)
Antigen retrieval step None
Permeabilization Yes - Tween 0.1%
Specification Eye
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2 · Temperature: 24°C
Fixative Paraformaldehyde

Onkar Sawant

Verified customer

提交于 Jan 28 2016

Thank you for contacting us.

The band size seems to be correct. This product has a predicted weight of 39kDa although you band seems a bit smaller this is common when using in real world settings as compared to predictions. Post translational...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this recommended product was not correct for your assay and for the inconvenience this has cau...

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