Anti-Rhodopsin抗体[RET-P1] (ab81702)

概述

  • 产品名称Anti-Rhodopsin抗体[RET-P1]
    参阅全部 Rhodopsin 一抗
  • 描述
    小鼠单克隆抗体[RET-P1] to Rhodopsin
  • 特异性ab81702 specifically labels the axons and synaptic pedicles of the rods.
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Membrane preparation from adult rat retina.

  • 阳性对照
    • Brain or retina.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 10mM PBS, pH 7.4
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明Purified from ascites fluid by Protein G chromatography.
  • 克隆单克隆
  • 克隆编号RET-P1
  • 同种型IgG1
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab81702 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
ICC/IF
  • 应用说明ICC/IF: Use at an assay dependent dilution.
    IHC-P: 1/25 - 1/50 for 60 minutes at room temperature. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 minutes.
    WB: Use at a concentration of 1 - 2 µg/ml for 2 hours at room temperature. Predicted molecular weight: 39 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.
    • 组织特异性Rod shaped photoreceptor cells which mediates vision in dim light.
    • 疾病相关Retinitis pigmentosa 4
      Night blindness, congenital stationary, autosomal dominant 1
    • 序列相似性Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.
    • 翻译后修饰Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
      Contains one covalently linked retinal chromophore.
    • 细胞定位Membrane. Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CSNBAD1 antibody
      • MGC138309 antibody
      • MGC138311 antibody
      • OPN 2 antibody
      • OPN2 antibody
      • opsd antibody
      • OPSD_HUMAN antibody
      • opsin 2 antibody
      • Opsin 2 rod pigment antibody
      • Opsin-2 antibody
      • Opsin2 antibody
      • Retinitis Pigmentosa 4 antibody
      • Retinitis pigmentosa 4 autosomal dominant antibody
      • RHO antibody
      • Rhodopsin antibody
      • RP 4 antibody
      • RP4 antibody
      see all

    Anti-Rhodopsin antibody [RET-P1] 图像

    • Formalin fixed, paraffin embedded human brain tissue stained with anti Rhodopsin (form of ab81702 containing BSA and azide) at a 1/25 dilution. Peroxidase conjugate and AEC chromogen were used. Note cytoplasmic staining of neurons.
    • anti Rhodopsin (form of ab81702 containing BSA and azide) at 2 µg/ml + human cells

      Predicted band size : 39 kDa
      Observed band size : 39 kDa

    Anti-Rhodopsin antibody [RET-P1] (ab81702)参考文献

    ab81702 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"