The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明ICC/IF: Use at an assay dependent dilution.
IHC-P: 1/25 - 1/50 for 60 minutes at room temperature. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 minutes.
WB: Use at a concentration of 1 - 2 µg/ml for 2 hours at room temperature. Predicted molecular weight: 39 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.
组织特异性Rod shaped photoreceptor cells which mediates vision in dim light.
疾病相关Retinitis pigmentosa 4 Night blindness, congenital stationary, autosomal dominant 1
序列相似性Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.
翻译后修饰Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region. Contains one covalently linked retinal chromophore.
细胞定位Membrane. Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia.
Formalin fixed, paraffin embedded human brain tissue stained with anti Rhodopsin (form of ab81702 containing BSA and azide) at a 1/25 dilution. Peroxidase conjugate and AEC chromogen were used. Note cytoplasmic staining of neurons.
Western blot - Rhodopsin antibody [RET-P1] - BSA and Azide free (ab81702)
anti Rhodopsin (form of ab81702 containing BSA and azide) at 2 µg/ml + human cells
Predicted band size : 39 kDa Observed band size : 39 kDa
Anti-Rhodopsin antibody [RET-P1] (ab81702)参考文献
has not yet been referenced specifically in any publications.