Anti-Rhodopsin抗体[RET-P1] (ab3267)


  • 产品名称Anti-Rhodopsin抗体[RET-P1]
    参阅全部 Rhodopsin 一抗
  • 描述
    小鼠单克隆抗体[RET-P1] to Rhodopsin
  • 经测试应用适用于: ICC/IF, Flow Cyt, IHC-P, WB, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human
  • 免疫原

    Membrane preparation from adult rat retina.

  • 阳性对照
    • IMR-5 cells Brain or retina tissue sections.
  • 常规说明

    Rhodopsin is a glycoprotein which is a visual pigment in mammals, birds, amphibians, and reptiles. The rhodopsin protein is present on the photoreceptor cells in the retina.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.40
    Constituents: 3% Sodium deoxycholate, 3% Triton-X-100, 0.3% Tris HCl, 15% Sodium chloride
  • Concentration information loading...
  • 纯度Protein G purified
  • Primary antibody说明Rhodopsin is a glycoprotein which is a visual pigment in mammals, birds, amphibians, and reptiles. The rhodopsin protein is present on the photoreceptor cells in the retina.
  • 克隆单克隆
  • 克隆编号RET-P1
  • 同种型IgG1
  • 研究领域


Our Abpromise guarantee covers the use of ab3267 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P 1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Staining protocol - boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 min.

WB Use at an assay dependent concentration. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).

Blot incubation - 2hrs at RT

IP Use at an assay dependent concentration. PubMed: 18974094


  • 功能Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.
  • 组织特异性Rod shaped photoreceptor cells which mediates vision in dim light.
  • 疾病相关Retinitis pigmentosa 4
    Night blindness, congenital stationary, autosomal dominant 1
  • 序列相似性Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.
  • 翻译后修饰Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
    Contains one covalently linked retinal chromophore.
  • 细胞定位Membrane. Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CSNBAD1 antibody
    • MGC138309 antibody
    • MGC138311 antibody
    • OPN 2 antibody
    • OPN2 antibody
    • opsd antibody
    • OPSD_HUMAN antibody
    • opsin 2 antibody
    • Opsin 2 rod pigment antibody
    • Opsin-2 antibody
    • Opsin2 antibody
    • Retinitis Pigmentosa 4 antibody
    • Retinitis pigmentosa 4 autosomal dominant antibody
    • RHO antibody
    • Rhodopsin antibody
    • RP 4 antibody
    • RP4 antibody
    see all

Anti-Rhodopsin antibody [RET-P1] 图像

  • ab3267 at a 1/100 dilution staining Rhosopsin in murine retina by IHC-Wholemount. Tissue was fixed in 4% paraformaldehyde and permeabilized using Triton. The secondary used was an Atto488 conjugated goat polyclonal used at a 1/200 dilution.
    Rods stained with ab3267 (green) in whole mount retina of young adult mouse (p21), actin stained using phalloidin (red).

    See Abreview

  • Predicted band size : 39 kDa

    Image from Chucair-Elliott AJ et al., J Biol Chem 287:24092-102 (2012); Fig 1.; doi: 10.1074/jbc.M112.378240; July 13, 2012, The Journal of Biological Chemistry, 287, 24092-24102.

  • ab3267 staining Rhodopsin in Rat eyeball tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 20 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500 in 1% BSA in PBS) for 1 hours at 25°C. A HRP-conjugated Goat anti-mouse IgG polyclonal undiluted was used as the secondary antibody.
    Rods and cones of retinal outter granular cells were stained positively for rhodopsin. In normal conditions, synthesized rhodopsin is predominatly stored in the cellular projects, not in the cell bodies.

    See Abreview

  • Overlay histogram showing Y79 cells stained with ab3267 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab3267, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Y79 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Rhodopsin antibody [RET-P1] (ab3267)参考文献

This product has been referenced in:
  • Tzameret A  et al. Transplantation of human bone marrow mesenchymal stem cells as a thin subretinal layer ameliorates retinal degeneration in a rat model of retinal dystrophy. Exp Eye Res 118:135-44 (2014). IHC-P . Read more (PubMed: 24239509) »
  • Wavre-Shapton ST  et al. Conditional ablation of the choroideremia gene causes age-related changes in mouse retinal pigment epithelium. PLoS One 8:e57769 (2013). ICC/IF ; Mouse . Read more (PubMed: 23460904) »

See all 10 Publications for this product

Product Wall

The concentration of lot GR93754 for ab3267 is 200 ug/ml.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (eyeball)
Specification eyeball
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH6
Permeabilization No
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Sep 28 2012

Thank you for your enquiry.

I can confirm that recent trials did not show any influence on product performance after an additional freeze thaw cycle and so we advise customers that they can store this antibody frozen at -20oC now.

Read More

Application IHC - Wholemount
Sample Mouse Tissue (retina)
Specification retina

Dr. Vincent Michel

Verified customer

提交于 Mar 26 2012

Application Immunohistochemistry free floating
Sample House Sparrow Cell (brain)
Specification brain

Dr. Gang Wang

Verified customer

提交于 Mar 01 2011

Thank you for your enquiry. It may indeed be possible to use antibodies in IHC-Fr (frozen sections) when they are already working in IHC-P. This is likely as the fact they work in IHC-P means they have the capacity to recognise the antigen in its na...

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Thank you for your enquiry. The antibody ab3267 has been tested in immunocytochemistry in IF in IMR-5 cells, unfortunately we do not have information about the dilution used but since the antibody worked at 4ug /ml for 30 min at RT in paraffin sections...

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This data is unknown.