Synthetic peptide: YEDSTHASIFTYT, corresponding to amino acids 14/26 of Mouse Green cones Opsin 1; and synthetic peptide corresponding to amino acids 18/30 of tiger red cones opsin 1 were mixed and used as immunogen.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/2000. Predicted molecular weight: 40 kDa. To minimise background staining, it is recommended to use higher content of detergent Tween 20 to dilute the antibody and during washing.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.
Rod shaped photoreceptor cells which mediates vision in dim light.
Retinitis pigmentosa 4 Night blindness, congenital stationary, autosomal dominant 1
Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.
Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region. Contains one covalently linked retinal chromophore.
Membrane. Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia.