Rat, Rabbit, Zebrafish, Zebra finch
Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human RHOC.
(Peptide available as
This antibody gave a positive signal in the following Lysates:
EBS E14 TG2A Day 3 Whole Cell, U2OS Whole Cell, MOLT4 Whole Cell and Human Brain Tissue.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 22 kDa).
Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers.
Belongs to the small GTPase superfamily. Rho family.
Information by UniProt
Aplysia RAS-related homolog 9 antibody
ARH 9 antibody
Western blot - Anti-RHOC antibody (ab64659)
All lanes :
Anti-RHOC antibody (ab64659) at 1 µg/ml
Lane 1 :
EBS E14 TG2A Day 3 (Mouse Pluripotent Embryonic Stem Cell) Whole Cell Lysate
Lane 2 :
U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lane 3 :
MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
Lane 4 :
Human brain tissue lysate - total protein (
Lysates/proteins at 10 µg per lane.
Secondary All lanes :
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
19 kDa (
why is the actual band size different from the predicted?
Additional bands at:
55 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - Anti-RHOC antibody (ab64659)
ICC/IF image of ab64659 stained HeLa cells. The cells were 100% methanol fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64659, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa cells at 5µg/ml.
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