概述

  • 产品名称
  • 描述
    兔多克隆抗体to RHOC
  • 经测试应用
    适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Zebrafish, Zebra finch
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human RHOC.

    (Peptide available as ab85816.)

  • 阳性对照
    • This antibody gave a positive signal in the following Lysates: EBS E14 TG2A Day 3 Whole Cell, U2OS Whole Cell, MOLT4 Whole Cell and Human Brain Tissue.

性能

应用

Our Abpromise guarantee covers the use of ab64659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 22 kDa).

靶标

  • 功能
    Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers.
  • 序列相似性
    Belongs to the small GTPase superfamily. Rho family.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Aplysia RAS-related homolog 9 antibody
    • ARH 9 antibody
    • ARH9 antibody
    • ARHC antibody
    • H9 antibody
    • MGC1448 antibody
    • MGC61427 antibody
    • Oncogene RHO H9 antibody
    • Ras homolog gene family member C antibody
    • RAS related homolog 9 antibody
    • RHO C antibody
    • Rho cDNA clone 9 antibody
    • Rho related GTP binding protein RhoC antibody
    • Rho-related GTP-binding protein RhoC antibody
    • rhoC antibody
    • RhoC GTPase antibody
    • RHOC_HUMAN antibody
    • RHOH 9 antibody
    • RHOH9 antibody
    • Small GTP binding protein RhoC antibody
    see all

图片

  • All lanes : Anti-RHOC antibody (ab64659) at 1 µg/ml

    Lane 1 : EBS E14 TG2A Day 3 (Mouse Pluripotent Embryonic Stem Cell) Whole Cell Lysate
    Lane 2 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
    Lane 3 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
    Lane 4 : Human brain tissue lysate - total protein (ab29466)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 22 kDa
    Observed band size : 19 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab64659 stained HeLa cells. The cells were 100% methanol fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64659, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa cells at 5µg/ml.

文献

This product has been referenced in:
  • Zimmerman NP  et al. Cyclic AMP regulates the migration and invasion potential of human pancreatic cancer cells. Mol Carcinog 54:203-15 (2015). Read more (PubMed: 24115212) »
  • Hauser AD  et al. The SmgGDS splice variant SmgGDS-558 is a key promoter of tumor growth and RhoA signaling in breast cancer. Mol Cancer Res 12:130-42 (2014). Read more (PubMed: 24197117) »

See all 3 Publications for this product

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