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Our Abpromise guarantee covers the use of ab59123 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.25 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 79 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
|ELISA||Use at an assay dependent concentration.
Titre using peptide based assay: 1:312500.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling RHOBTB1 with ab59123 at a concentration of 4-8µg/ml.