概述

  • 产品名称Anti-RHOBTB1抗体
    参阅全部 RHOBTB1 一抗
  • 描述
    兔多克隆抗体to RHOBTB1
  • 经测试应用适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide (Human)

  • 阳性对照
    • Jurkat cells; intestine.

性能

应用

Our Abpromise guarantee covers the use of ab59123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 79 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:312500.

靶标

Anti-RHOBTB1 antibody 图像

  • Anti-RHOBTB1 antibody (ab59123) at 0.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 79 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling RHOBTB1 with ab59123 at a concentration of 4-8µg/ml.

Anti-RHOBTB1 antibody (ab59123)参考文献

This product has been referenced in:
  • Heasman SJ  et al. Coordinated RhoA signaling at the leading edge and uropod is required for T cell transendothelial migration. J Cell Biol 190:553-63 (2010). WB ; Human . Read more (PubMed: 20733052) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry.

I can confirm that in testing of this antibody, the following antigen retrieval procedure was used:

- Retrieve epitopes by incubating the sections at 98-100° C in a microwave in 0.01 M citrate buffe...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"